Miranda Alexandre F, Gallo Carla B M, De Souza Diogo B, Costa Waldemar S, Sampaio Francisco J B
Urogenital Research Unit, Rio de Janeiro State University, Rio de Janeiro, Brazil.
J Androl. 2012 Nov-Dec;33(6):1224-32. doi: 10.2164/jandrol.112.017012. Epub 2012 Jul 12.
The objective of the study was to evaluate, through quantitative methods, the structural alterations in the corpora cavernosa of rats submitted to orchiectomy as well as the role of late hormone replacement in overturning the possible structural alterations. Twenty-five male rats were assigned into 5 groups with 5 animals each and treated as follows: ORCHIEC-1 = submitted to orchiectomy and sacrificed after 1 month; C-1 = control group sacrificed after 1 month; ORCHIEC-2 = submitted to orchiectomy and sacrificed after 2 months; C-2 = control group sacrificed after 2 months; and T = submitted to orchiectomy, underwent testosterone replacement with testosterone undecanoate (100 mg/kg) after 1 month, and sacrificed after 1 month of hormonal replacement. Smooth muscle, collagen, and elastic system fibers of penile corpora cavernosa were quantified. There was a significant decrease in the absolute values of smooth muscle, sinusoidal space, and total area of corpora cavernosa after 2 months in the castrated group when compared with controls. Overall, regarding density, no significant differences were observed among the groups. The hormonal replacement with testosterone was able to reverse the alterations observed. The method used for this research allowed demonstrating that absolute values are reliable to quantify the structural alterations of corpora cavernosa structures. The results suggest that hormonal replacement, even when instituted at a late stage, is effective in reversing the corpora cavernosa's structural alterations produced by castration.
本研究的目的是通过定量方法评估接受睾丸切除的大鼠海绵体的结构改变，以及晚期激素替代在扭转可能的结构改变中的作用。将25只雄性大鼠分为5组，每组5只，处理如下：ORCHIEC - 1组 = 接受睾丸切除并在1个月后处死；C - 1组 = 对照组，在1个月后处死；ORCHIEC - 2组 = 接受睾丸切除并在2个月后处死；C - 2组 = 对照组，在2个月后处死；T组 = 接受睾丸切除，1个月后用十一酸睾酮（100 mg/kg）进行睾酮替代，在激素替代1个月后处死。对阴茎海绵体的平滑肌、胶原蛋白和弹性系统纤维进行定量分析。与对照组相比，去势组在2个月后海绵体的平滑肌绝对值、窦状间隙和总面积显著降低。总体而言，就密度而言，各组之间未观察到显著差异。睾酮激素替代能够逆转所观察到的改变。本研究使用的方法能够证明绝对值对于量化海绵体结构的改变是可靠的。结果表明，即使在晚期进行激素替代，也能有效逆转去势引起的海绵体结构改变。
