Department of Prosthodontics and Dental Materials, Medical Faculty, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074, Aachen, Germany.
Lasers Med Sci. 2013 Jan;28(1):303-9. doi: 10.1007/s10103-012-1148-6. Epub 2012 Jul 12.
Several implant surface debridement methods have been reported for the treatment of peri-implantitis, however, some of them can damage the implant surface or promote bacterial resistance. Photodynamic therapy (PDT) is a new treatment option for peri-implantitis. The aim of this in vitro study was to analyze implant surface decontamination by means of PDT. Sixty implants were equally distributed (n = 10) into four groups and two subgroups. In group G1 there was no decontamination, while in G2 decontamination was performed with chlorhexidine. G3 (PDT - laser + dye) and G4 (laser, without dye) were divided into two subgroups each; with PDT performed for 3 min in G3a and G4a, and for 5 min in G3b and G4b. After 5 min in contact with methylene blue dye (G3), the implants were irradiated (G3 and G4) with a low-level laser (GaAlAs, 660 nm, 30 mW) for 3 or 5 min (7.2 and 12 J). After the dilutions, culture media were kept in an anaerobic atmosphere for 1 week, and then colony forming units were counted. There was a significant difference (p < 0.001) between G1 and the other groups, and between G4 in comparison with G2 and G3. Better decontamination was obtained in G2 and G3, with no statistically significant difference between them. The results of this study suggest that photodynamic therapy can be considered an efficient method for reducing bacteria on implant surfaces, whereas laser irradiation without dye was less efficient than PDT.
已有多种种植体表面清创方法被报道用于治疗种植体周围炎，然而，其中一些方法可能会损伤种植体表面或促进细菌耐药性。光动力疗法（PDT）是一种治疗种植体周围炎的新方法。本体外研究旨在分析 PDT 对种植体表面的去污效果。将 60 个种植体平均分为 4 组（n = 10）和 2 个亚组。在 G1 组中没有进行去污处理，而在 G2 组中用洗必泰进行去污处理。G3（PDT-激光+染料）和 G4（激光，无染料）各分为 2 个亚组；G3a 和 G4a 中进行 3 分钟 PDT，G3b 和 G4b 中进行 5 分钟 PDT。在与亚甲基蓝染料接触 5 分钟后（G3），用低水平激光（GaAlAs，660nm，30mW）对种植体进行 3 或 5 分钟（7.2 和 12J）照射（G3 和 G4）。稀释后，将培养介质在厌氧气氛中保持 1 周，然后计算菌落形成单位。G1 与其他组之间以及 G4 与 G2 和 G3 之间存在显著差异（p<0.001）。G2 和 G3 组的去污效果更好，它们之间无统计学差异。本研究结果表明，光动力疗法可被认为是减少种植体表面细菌的有效方法，而无染料的激光照射效率低于 PDT。
