Department of Neurology and Neurosurgery, Stem Cell Research Laboratory, University of Warmia and Mazury, ul. Warszawska 30, 10-082 Olsztyn, Poland.
J Mol Neurosci. 2012 Nov;48(3):766-76. doi: 10.1007/s12031-012-9855-9. Epub 2012 Jul 12.
The SLC30 family of divalent cation transporters is thought to be involved in the transport of zinc in a variety of cellular pathways. Zinc transporter 3 (ZnT3) is involved in the transport of zinc into synaptic vesicles or intracellular organelles. As the presence of ZnT3 immunoreactive neurons has recently been reported in both the central and peripheral nervous systems of the rat, the present study was aimed at disclosing the presence of a zinc-enriched neuron enteric population in the porcine duodenum to establish a preliminary insight into their neurochemical coding. Double- and triple-immunofluorescence labeling of the porcine duodenum for ZnT3 with the pan-neuronal marker (PGP 9.5), substance P, somatostatin, vasoactive intestinal peptide (VIP), nitric oxide synthase (NOS), leu-enkephalin, vesicular acetylcholine transporter (VAChT), neuropeptide Y, galanin (GAL), and calcitonin gene-related peptide were performed. Immunohistochemistry revealed that approximately 35, 43, and 48 % of all PGP9.5-postive neurons in the myenteric (MP), outer submucous (OSP), and inner submucous (ISP) plexuses, respectively, of the porcine duodenum were simultaneously ZnT3(+). In the present study, ZnT3(+) neurons coexpressed a broad spectrum of active substances, but co-localization patterns unique to the plexus were studied. In the ISP, all ZnT3(+) neurons were VAChT positive, and the largest populations among these cells formed ZnT3(+)/VAChT(+)/GAL(+) and ZnT3(+)/VAChT(+)/VIP(+) cells. In the OSP and MP, the numbers of ZnT3(+)/VAChT(+) neurons were two times smaller, and substantial subpopulations of ZnT3(+) neurons in both these plexuses formed ZnT3(+)/NOS(+) cells. The large population of ZnT3(+) neurons in the porcine duodenum and a broad spectrum of active substances which co-localize with this peptide suggest that ZnT3 takes part in the regulation of various processes in the gut both in normal physiology and during pathological processes.
SLC30 家族的二价阳离子转运体被认为参与多种细胞途径中的锌转运。锌转运体 3（ZnT3）参与锌向突触小泡或细胞内细胞器的转运。由于最近在大鼠的中枢和周围神经系统中均报道了 ZnT3 免疫反应性神经元的存在，因此本研究旨在揭示猪十二指肠中富含锌的神经元群体的存在，以初步了解其神经化学编码。用泛神经元标志物（PGP 9.5）、P 物质、生长抑素、血管活性肠肽（VIP）、一氧化氮合酶（NOS）、亮啡肽、囊泡乙酰胆碱转运体（VAChT）、神经肽 Y、胆囊收缩素（GAL）和降钙素基因相关肽对猪十二指肠进行双重和三重免疫荧光标记ZnT3。免疫组织化学显示，猪十二指肠肌间（MP）、外黏膜下（OSP）和内黏膜下（ISP）神经丛中约 35%、43%和 48%的 PGP9.5 阳性神经元同时为 ZnT3(+)。在本研究中，ZnT3(+)神经元共表达了广泛的活性物质，但研究了独特于神经丛的共定位模式。在 ISP 中，所有 ZnT3(+)神经元均为 VAChT 阳性，这些细胞中最大的群体形成了 ZnT3(+)/VAChT(+)/GAL(+)和 ZnT3(+)/VAChT(+)/VIP(+)细胞。在 OSP 和 MP 中，ZnT3(+)/VAChT(+)神经元的数量减少了两倍，这两个神经丛中 ZnT3(+)神经元的大量亚群形成了 ZnT3(+)/NOS(+)细胞。猪十二指肠中大量的 ZnT3(+)神经元和与该肽共定位的广泛活性物质表明，ZnT3 参与了肠道在正常生理和病理过程中各种过程的调节。
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