Wojtkiewicz Joanna, Makowska Krystyna, Bejer-Olenska Ewa, Gonkowski Sławomir
Department of Pathophysiology, Faculty of Medical Sciences, Warszawska Str. 30, 10-082, Olsztyn, Poland.
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Str. 13, 10-718, Olsztyn, Poland.
J Mol Neurosci. 2017 Mar;61(3):315-324. doi: 10.1007/s12031-016-0854-0. Epub 2016 Oct 30.
Zinc transporter 3 (ZnT3), a member of the SLC 30 zinc transporter family, is involved in the transport of zinc ions from the cytoplasm into synaptic vesicles or intracellular organelles. The aim of the present study was to investigate for the first time the percentage of ZnT3-like immunoreactive (ZnT3-LI) neurons in the enteric nervous system (ENS) of the porcine esophagus and denotation of their neurochemical coding. Routine double- and triple-immunofluorescence labeling of cervical, thoracic, and abdominal fragments of esophagus for ZnT3 with protein gene product (PGP 9.5; used as pan-neuronal marker), nitric oxide synthase (NOS), somatostatin, vasoactive intestinal peptide (VIP), vesicular acetylcholine transporter (VAChT), neuropeptide Y (NPY), and galanin (GAL) was performed. The percentage of ZnT3-LI neurons in myenteric ganglia amounted to 50.2 ± 4.7, 63.4 ± 8.3, and 77.1 ± 1.1 % of all PGP 9.5-like immunoreactive neuronal cells in cervical, thoracic, and abdominal esophagus, respectively. In submucous ganglia, these values in particular parts of esophagus amounted to 46.3 ± 6.3, 81.0 ± 8.1, and 74.4 ± 4.4 %. Znt3 co-localized mainly with VAChT, NPY, GAL, NOS, and VIP, but the degree of co-localization depended on the "kind" of enteric ganglia and part of esophagus studied. The obtained results suggest that both ZnT3 and zinc ions may play important and various roles in the neuronal regulation of esophageal functions.
锌转运体3（ZnT3）是溶质载体家族30（SLC 30）锌转运体家族的成员之一，参与锌离子从细胞质转运到突触小泡或细胞内细胞器的过程。本研究的目的是首次调查猪食管肠神经系统（ENS）中锌转运体3样免疫反应性（ZnT3-LI）神经元的百分比及其神经化学编码。对食管颈段、胸段和腹段进行常规双重和三重免疫荧光标记，以检测ZnT3与蛋白基因产物（PGP 9.5；用作泛神经元标记物）、一氧化氮合酶（NOS）、生长抑素、血管活性肠肽（VIP）、囊泡乙酰胆碱转运体（VAChT）、神经肽Y（NPY）和甘丙肽（GAL）。肌间神经节中ZnT3-LI神经元在颈段、胸段和腹段食管中分别占所有PGP 9.5样免疫反应性神经元细胞的50.2±4.7%、63.4±8.3%和77.1±1.1%。在黏膜下神经节中，食管特定部位的这些值分别为46.3±6.3%、81.0±8.1%和74.4±4.4%。ZnT3主要与VAChT、NPY、GAL、NOS和VIP共定位，但共定位程度取决于所研究的肠神经节“类型”和食管部位。所得结果表明，ZnT3和锌离子可能在食管功能的神经元调节中发挥重要且多样的作用。
