Carmona-Ribeiro Ana Maria
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
Methods Mol Biol. 2012;906:283-94. doi: 10.1007/978-1-61779-953-2_22.
Biomimetic nanoparticles are hybrid nanostructures in which the uppermost layer is similar to a cell membrane. This report provides instructions for the preparation and physical characterization of three different types of biomimetic nanoparticles: (1) polystyrene sulfate nanoparticles covered with one cationic dioctadecyldimethylammonium bilayer; (2) silica nanoparticles covered with one neutral phosphatidylcholine bilayer; (3) miconazole particles covered with one anionic dihexadecylphosphate (DHP) bilayer. These examples provide the basis for the preparation and characterization of novel nanoparticles from hydrophobic or hydrophilic and organic or inorganic nanoparticle cores covered with functional outer layers. The major concepts and technical details for obtaining the optimal lipid coverage of supporting cores and for nanoparticle characterization are discussed.
仿生纳米粒子是一种杂化纳米结构，其最外层类似于细胞膜。本报告提供了三种不同类型仿生纳米粒子的制备方法及物理特性说明：（1）覆盖有一层阳离子二辛基二甲基铵双层的聚苯乙烯硫酸纳米粒子；（2）覆盖有一层中性磷脂酰胆碱双层的二氧化硅纳米粒子；（3）覆盖有一层阴离子二己基磷酸酯（DHP）双层的咪康唑粒子。这些实例为从覆盖有功能外层的疏水或亲水、有机或无机纳米粒子核制备和表征新型纳米粒子提供了基础。讨论了获得支撑核最佳脂质覆盖率及纳米粒子表征的主要概念和技术细节。
