Key Laboratory of Pulmonary Diseases of Health Ministry, Department of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
PLoS One. 2012;7(7):e40450. doi: 10.1371/journal.pone.0040450. Epub 2012 Jul 9.
The objective of the present study was to investigate the presence of interleukin (IL)-27 in pleural effusions and to evaluate the diagnostic significance of pleural IL-27. The concentrations of IL-27 were determined in pleural fluids and sera from 68 patients with tuberculous pleural effusion, 63 malignant pleural effusion, 22 infectious pleural effusion, and 21 transudative pleural effusion. Flow cytometry was used to identify which pleural cell types expressed IL-27. It was found that the concentrations of pleural IL-27 in tuberculous group were significantly higher than those in malignant, infectious, and transudative groups, respectively. Pleural CD4(+) T cells, CD8(+) T cells, NK cells, NKT cells, B cells, monocytes, macrophages, and mesothelial cells might be the cell sources for IL-27. IL-27 levels could be used for diagnostic purpose for tuberculous pleural effusion, with the cut off value of 1,007 ng/L, IL-27 had a sensitivity of 92.7% and specificity of 99.1% for differential diagnosing tuberculous pleural effusion from non-tuberculous pleural effusions. Therefore, compared to non-tuberculous pleural effusions, IL-27 appeared to be increased in tuberculous pleural effusion. IL-27 in pleural fluid is a sensitive and specific biomarker for the differential diagnosing tuberculous pleural effusion from pleural effusions with the other causes.
本研究旨在探讨白细胞介素 (IL)-27 在胸腔积液中的存在，并评估胸腔 IL-27 的诊断意义。测定了 68 例结核性胸腔积液、63 例恶性胸腔积液、22 例感染性胸腔积液和 21 例渗出性胸腔积液患者胸腔液和血清中的 IL-27 浓度。采用流式细胞术鉴定表达 IL-27 的胸腔细胞类型。结果发现，结核组胸腔 IL-27 浓度明显高于恶性、感染性和渗出性组。胸腔 CD4(+)T 细胞、CD8(+)T 细胞、NK 细胞、NKT 细胞、B 细胞、单核细胞、巨噬细胞和间皮细胞可能是 IL-27 的细胞来源。IL-27 水平可用于结核性胸腔积液的诊断目的，以 1007ng/L 为截断值，IL-27 对鉴别结核性胸腔积液与非结核性胸腔积液的敏感性为 92.7%，特异性为 99.1%。因此，与非结核性胸腔积液相比，结核性胸腔积液中 IL-27 似乎增加。胸腔液中的 IL-27 是鉴别结核性胸腔积液与其他原因胸腔积液的一种敏感且特异的生物标志物。
