Tsujibo H, Sakamoto T, Miyamoto K, Kusano G, Ogura M, Hasegawa T, Inamori Y
Osaka University of Pharmaceutical Sciences, Japan.
Chem Pharm Bull (Tokyo). 1990 Aug;38(8):2299-300. doi: 10.1248/cpb.38.2299.
An alkalophilic actinomycete, strain OPC-553 regarded as Nocardiopsis dassonvillei subsp. prasina, produced the cytotoxic substance, TS-1, which showed a marked inhibitory activity against L5178Y mouse leukemic cell in vitro. The cytotoxicity of TS-1 on this cell was very strong and its ID50 was 0.018 micrograms/ml. Through direct comparison of its spectral data with those of an authentic sample, TS-1 was identified as the antifungal antibiotic, kalafungin, already isolated from the culture broth of Streptomyces tanashiensis. However, the isolation of kalafungin from an alkalophilic actinomycete and its cytotoxicity are reported for the first time in this paper.
一种嗜碱放线菌，菌株OPC-553被认为是达松维尔诺卡氏菌嗜绿亚种，产生了细胞毒性物质TS-1，该物质在体外对L5178Y小鼠白血病细胞显示出显著的抑制活性。TS-1对该细胞的细胞毒性非常强，其半数抑制浓度为0.018微克/毫升。通过将其光谱数据与正品样品的光谱数据直接比较，TS-1被鉴定为抗真菌抗生素卡拉芬净，该抗生素已从塔纳链霉菌的培养液中分离得到。然而，本文首次报道了从嗜碱放线菌中分离出卡拉芬净及其细胞毒性。
