National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing, 210093, P. R. China.
J Phys Chem B. 2012 Aug 9;116(31):9432-8. doi: 10.1021/jp305141r. Epub 2012 Aug 1.
The molecular-level interactions of an antimicrobial peptide melittin with supported membrane were studied by the combination of dissipative quartz crystal microbalance (QCM-D) experiments and computer simulations. We found the response behavior of lipids upon peptide adsorption greatly influence their interactions. The perturbance and reorientation of the lipid in liquid phase facilitate the insertion of melittin in a trans-membrane way, but in solid phase, asymmetrical membrane disruption happens. Apart from the lipid state, the local peptide-to-lipid ratio also affects the insertion capacity of melittin. When the local peptide number density is high, adjacent peptides can cooperatively penetrate into the membrane. This observation explains the occurrence of the conventional "carpet" mechanism.
通过耗散石英晶体微天平（QCM-D）实验和计算机模拟相结合的方法，研究了抗菌肽蜂毒素与支撑膜之间的分子级相互作用。我们发现，肽吸附时脂质的响应行为会极大地影响它们的相互作用。在液相中，脂质的扰动和重排有助于蜂毒素以跨膜的方式插入，但在固相中，不对称的膜破裂会发生。除了脂质状态外，局部肽与脂质的比例也会影响蜂毒素的插入能力。当局部肽数密度较高时，相邻的肽可以协同地穿透膜。这一观察结果解释了常规“地毯”机制的发生。
