Faculty of Geography, Earth and Environmental Sciences, University of Birmingham, UK.
Chemosphere. 2013 Jan;90(2):164-9. doi: 10.1016/j.chemosphere.2012.06.021. Epub 2012 Jul 12.
The single and joint effects of chromium (Cr) and benzo (a) pyrene (B (a) P) on the seed germination and the elongation of root and shoot of Lolium perenne were investigated. Seed germination represents the first important step to effective phytoremediation. Young seedlings may be susceptible to PAH and heavy metal contaminants. The results showed that in solution, increasing concentration of Cr could inhibit the germination rate as well as root and shoot elongation of L. perenne. Also, the increasing concentration of B (a) P (1-4 mg L(-1)) could accelerate the germination rate. The joint toxicity of Cr and B (a) P showed that increasing concentration of Cr and B (a) P had significant antagonistic effect on the germination rate of L. perenne. In the single factor experiments and joint effect tests of Cr and B (a) P on the seedling growth, the root and shoot elongation were inhibited significantly (p<0.05) for higher concentration of Cr whereas increasing concentration of B (a) P accelerated the shoot elongation. There were significant relationships between the concentration of pollutants and root and shoot elongation (p<0.05). Higher concentration of B (a) P with low concentration of Cr had significant antagonistic effect on shoot and root elongation of L. perenne in solution tests. Also, low concentration of B (a) P with increasing concentration of Cr had a significant synergistic effect on shoot elongation. The toxicity effects of Cr and B (a) P to seed germination, root and shoot elongation are-root elongation>shoot elongation>germination rate.
研究了铬（Cr）和苯并（a）芘（B（a）P）对黑麦草种子萌发和根伸长及芽伸长的单一和联合作用。种子萌发是有效植物修复的第一步。幼苗可能容易受到多环芳烃和重金属污染物的影响。结果表明，在溶液中，Cr 浓度的增加会抑制黑麦草的发芽率以及根和芽的伸长。而且，B（a）P（1-4mg/L）浓度的增加会加速发芽率。Cr 和 B（a）P 的联合毒性表明，Cr 和 B（a）P 浓度的增加对黑麦草的发芽率有显著的拮抗作用。在 Cr 和 B（a）P 对幼苗生长的单因素实验和联合作用试验中，较高浓度的 Cr 显著抑制根和芽的伸长（p<0.05），而 B（a）P 浓度的增加则加速了芽的伸长。污染物浓度与根和芽伸长之间存在显著关系（p<0.05）。在溶液试验中，高浓度的 B（a）P 与低浓度的 Cr 对黑麦草的芽和根伸长有显著的拮抗作用。而且，低浓度的 B（a）P 与 Cr 浓度的增加对芽伸长有显著的协同作用。Cr 和 B（a）P 对种子萌发、根和芽伸长的毒性效应为根伸长>芽伸长>发芽率。
