应用高分辨率熔解曲线分析马来亚裔糖尿病视网膜病变患者 SLC2A1 26177A/G 基因多态性的研究。
Investigation of SLC2A1 26177A/G gene polymorphism via high resolution melting curve analysis in Malaysian patients with diabetic retinopathy.
机构信息
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
出版信息
J Diabetes Complications. 2012 Sep-Oct;26(5):388-92. doi: 10.1016/j.jdiacomp.2012.05.014. Epub 2012 Jul 12.
PURPOSE
In this study, we aimed to investigate the possible association between SLC2A1 26177A/G polymorphism and diabetic retinopathy (DR) in Malaysian patients with type 2 diabetes.
METHODS
Genomic DNA was extracted from 211 Malaysian type 2 diabetic patients (100 without retinopathy [DNR], 111 with retinopathy) and 165 healthy controls. A high resolution melting assay developed in this study was used to detect SLC2A1 26177A/G polymorphism followed by statistical analysis.
RESULTS
A statistically significant difference in 26177G minor allele frequency between healthy controls (19.7 %) and total patient group (26.1 %) (p<0.05, Odd ratio = 1.437, 95% Confidence interval = 1.015-2.035) as well as between healthy controls (19.7 %) and DNR patients (27.5%) (p<0.05, Odd ratio = 1.546, 95% Confidence interval = 1.024-2.336) was shown in this study. However, when compared between DR and DNR patients, there was no significant difference (p>0.05).
CONCLUSIONS
This is the first study which shows that SLC2A1 26177G allele is associated with type 2 diabetes in Malaysian population but not with DR.
目的
在这项研究中，我们旨在探讨 SLC2A1 26177A/G 多态性与马来西亚 2 型糖尿病患者糖尿病视网膜病变（DR）之间的可能关联。
方法
从 211 名马来西亚 2 型糖尿病患者（100 名无视网膜病变[DNR]，111 名有视网膜病变）和 165 名健康对照中提取基因组 DNA。本研究开发了一种高分辨率熔解分析来检测 SLC2A1 26177A/G 多态性，然后进行统计分析。
结果
在健康对照组（19.7%）和总患者组（26.1%）（p<0.05，优势比=1.437，95%置信区间=1.015-2.035）以及健康对照组（19.7%）和 DNR 患者（27.5%）（p<0.05，优势比=1.546，95%置信区间=1.024-2.336）之间，26177G 小等位基因频率存在统计学显著差异。然而，在 DR 和 DNR 患者之间进行比较时，没有显著差异（p>0.05）。
结论
这是第一项表明 SLC2A1 26177G 等位基因与马来西亚人群 2 型糖尿病相关但与 DR 无关的研究。
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