Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 Hwayangdong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Res Vet Sci. 2012 Dec;93(3):1173-8. doi: 10.1016/j.rvsc.2012.06.004. Epub 2012 Jul 15.
Bacteriophage ΦCJ07 with broad host ranges for Salmonella strains isolated from sewage effluent were used to reduce Salmonella Enteritidis (SE) infection in chickens. One-day-old chicks challenged with 5×10(7) colony-forming units/bird of SE were cohabitated with contact chicks and treated with three concentrations (10(5), 10(7) and 10(9) plaque forming units (PFU)/g) of bacteriophage prepared as a feed additive for 21days after challenge. Salmonella in the intestine was quantified and environmental contamination level was examined at 1, 2 and 3weeks after challenge. All treatments reduced intestinal SE colonization in challenged and contact chickens and reduced the environmental contamination level, but the reductions produced by 10(7) and 10(9)PFU/g of bacteriophage were significant (P<0.05) as compared with untreated controls. In addition, seven out of 10 (70%) contact chickens treated with 10(9)PFU/g of bacteriophage had no detectable intestinal Salmonella at 3weeks after treatment, suggesting that bacteriophage therapy significantly prevented the horizontal transmission of SE. These results provide important insights into preventive and control strategies against SE infection in poultry and indicate that the use of bacteriophage could reduce the incidence of Salmonella food poisoning.
使用具有广泛宿主范围的噬菌体 ΦCJ07 来减少鸡中的肠炎沙门氏菌（SE）感染，该噬菌体可以从污水中分离到。用 5×10(7)个菌落形成单位/只的 SE 对 1 日龄雏鸡进行攻毒，并用接触鸡共同饲养，并在攻毒后 21 天用三种浓度（10(5)、10(7)和 10(9)噬菌斑形成单位 (PFU)/g）的噬菌体作为饲料添加剂进行处理。在攻毒后 1、2 和 3 周时定量检测肠道中的沙门氏菌，并检查环境污染物水平。所有处理均减少了攻毒和接触鸡的肠道 SE 定植，并降低了环境污染物水平，但与未处理对照组相比，噬菌体 10(7)和 10(9)PFU/g 的处理效果更为显著（P<0.05）。此外，用 10(9)PFU/g 的噬菌体处理的 10 只接触鸡中有 7 只（70%）在处理后 3 周时肠道内没有检测到沙门氏菌，表明噬菌体疗法显著防止了 SE 的水平传播。这些结果为禽类 SE 感染的预防和控制策略提供了重要的见解，并表明噬菌体的使用可以降低沙门氏菌食物中毒的发生率。
