UCD School of Medicine and Medical Science, Obstetrics and Gynaecology, University College Dublin, National Maternity Hospital, Dublin 2, Ireland.
Reprod Biomed Online. 2012 Sep;25(3):322-8. doi: 10.1016/j.rbmo.2012.05.001. Epub 2012 May 23.
The aim of this study was to compare the efficacy of endometrial aspiration biopsy (EAB) with the more traditional dilatation and curettage (D&C) for the procurement of lymphoid progenitor cells and uterine natural killer (NK) populations in endometrial tissue. This prospective observational study conducted in a tertiary referral university hospital examined endometrium obtained from 32 women admitted for laparoscopic gynaecological procedures. Each participant had endometrium sampled using both EAB and D&C. Both methods were assessed as a source of uterine NK and lymphoid progenitor cells. Similar proportions of mature CD45+CD56+ NK cells (range 25.4-36.2%) and CD45+CD34+ lymphoid progenitors (range 1.2-2.0%) were found in tissue obtained using both EAB and D&C. These cells were adequate for flow cytometric analysis, magnetic bead separation and culture. Colony formation by the CD34+ population demonstrated maturational potential. Tissues obtained via endometrial biopsy and D&C are equivalent, by analysis of uterine NK and lymphoid progenitor cells. The aim of this study was to compare two methods of endometrial sampling - endometrial aspiration biopsy and traditional dilatation and curettage - for the procurement of haematopoietic stem cells and uterine natural killer (NK) populations in endometrial tissue. Thirty-two women who had gynaecological procedures in a tertiary referral hospital participated in this study and had endometrial tissue collected via both methods. Similar populations of mature NK cells and haematopoietic stem cells were found in tissue obtained using both endometrial aspiration biopsy and dilatation and curettage. Tissue obtained via endometrial aspiration biopsy was adequate for the culture and growth of haematopoietic stem cells. We conclude that tissue obtained via endometrial biopsy and dilatation and curettage is equivalent, by analysis of uterine NK and haematopoietic stem cells using flow cytometry. This has implications for further more extensive study of endometrial haematopoietic stem cell populations, as these samples can be collected in a clinical setting without a requirement for general anaesthesia.
本研究旨在比较子宫内膜抽吸活检（EAB）与更传统的扩张刮宫术（D&C）在获取子宫内膜组织中的淋巴祖细胞和子宫自然杀伤（NK）细胞群体方面的疗效。这项在三级转诊大学医院进行的前瞻性观察性研究检查了 32 名因腹腔镜妇科手术入院的女性的子宫内膜。每位参与者均通过 EAB 和 D&C 两种方法采集子宫内膜样本。这两种方法均被评估为子宫 NK 和淋巴祖细胞的来源。通过 EAB 和 D&C 获得的组织中均发现成熟的 CD45+CD56+NK 细胞（范围 25.4-36.2%）和 CD45+CD34+淋巴祖细胞（范围 1.2-2.0%）比例相似。这些细胞足以进行流式细胞术分析、磁珠分离和培养。CD34+细胞群体的集落形成显示出成熟潜力。通过子宫内膜活检和 D&C 获得的组织在分析子宫 NK 和淋巴祖细胞方面是等效的。本研究旨在比较两种子宫内膜取样方法——子宫内膜抽吸活检和传统的扩张刮宫术——用于获取子宫内膜组织中的造血干细胞和子宫自然杀伤（NK）细胞群体。32 名在三级转诊医院接受妇科手术的女性参与了这项研究，并通过两种方法采集了子宫内膜组织。通过子宫内膜抽吸活检和扩张刮宫术获得的组织中均发现了成熟 NK 细胞和造血干细胞的相似群体。通过子宫内膜抽吸活检获得的组织足以用于造血干细胞的培养和生长。我们得出结论，通过流式细胞术分析，使用子宫内膜抽吸活检和扩张刮宫术获得的组织在子宫 NK 和造血干细胞方面是等效的。这对进一步更广泛地研究子宫内膜造血干细胞群体具有重要意义，因为这些样本可以在临床环境中收集，而无需全身麻醉。
