Lynch L, Golden-Mason L, Eogan M, O'Herlihy C, O'Farrelly Cliona
Education and Research Centre, St Vincent's University Hospital, Dublin, Ireland.
Hum Reprod. 2007 Apr;22(4):919-26. doi: 10.1093/humrep/del456. Epub 2007 Jan 5.
Uterine lymphoid cell repertoires are specialized in order to meet the twin demands of successful pregnancy and local immunosurveillance. The possibility that some of these populations might differentiate locally from progenitor cells has been proposed.
Endometrial tissue from women with a history of infertility as well as fertile controls was examined for haematopoietic stem cells (HSCs) and lymphoid progenitors using three-colour flow cytometry.
Significant populations of phenotypic HSCs (CD34+ CD45+ ) were detected in all samples, a high proportion of which co-expressed the differentiation marker CD45RA (45.7%), indicating ongoing differentiation. Almost 30% of uterine HSCs co-expressed CD56 and 44% co-expressed CD7, suggesting the presence of lymphoid progenitors. Small proportions expressed CD127 and CD122, receptors for interleukin (IL)-7 and IL-15, respectively. HSC numbers were similar in the endometrial samples from fertile and infertile women. However, the proportion co-expressing the natural killer (NK) antigen CD56 was significantly increased compared with HSCs found in the endometrium of fertile controls (P = 0.002).
This is the first demonstration of cells with an HSC phenotype in the human endometrium, and increased proportions of NK progenitors in endometrium of women with infertility suggests a dysregulation of this pathway that may contribute to infertility.
子宫淋巴细胞库具有特殊性，以满足成功妊娠和局部免疫监视的双重需求。有人提出这些细胞群体中的一些可能在局部由祖细胞分化而来。
使用三色流式细胞术检测有不孕史女性以及生育能力正常女性的子宫内膜组织中的造血干细胞（HSC）和淋巴祖细胞。
在所有样本中均检测到大量表型为HSC（CD34+ CD45+）的细胞，其中很大一部分共表达分化标志物CD45RA（45.7%），表明正在进行分化。近30%的子宫HSC共表达CD56，44%共表达CD7，提示存在淋巴祖细胞。小部分细胞分别表达白细胞介素（IL）-7和IL-15的受体CD127和CD122。生育能力正常和不孕女性的子宫内膜样本中HSC数量相似。然而，与生育能力正常女性子宫内膜中的HSC相比，共表达自然杀伤（NK）抗原CD56的比例显著增加（P = 0.002）。
这是首次在人子宫内膜中证实具有HSC表型的细胞，不孕女性子宫内膜中NK祖细胞比例增加表明该途径失调，这可能导致不孕。
