Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Drive, Madison, WI 53706, USA.
Insect Biochem Mol Biol. 2012 Oct;42(10):729-38. doi: 10.1016/j.ibmb.2012.06.005. Epub 2012 Jul 11.
Hemocytes are integral components of mosquito immune mechanisms such as phagocytosis, melanization, and production of antimicrobial peptides. However, our understanding of hemocyte-specific molecular processes and their contribution to shaping the host immune response remains limited. To better understand the immunophysiological features distinctive of hemocytes, we conducted genome-wide analysis of hemocyte-enriched transcripts, and examined how tissue-enriched expression patterns change with the immune status of the host. Our microarray data indicate that the hemocyte-enriched trascriptome is dynamic and context-dependent. Analysis of transcripts enriched after bacterial challenge in circulating hemocytes with respect to carcass added a dimension to evaluating infection-responsive genes and immune-related gene families. We resolved patterns of transcriptional change unique to hemocytes from those that are likely shared by other immune responsive tissues, and identified clusters of genes preferentially induced in hemocytes, likely reflecting their involvement in cell type specific functions. In addition, the study revealed conserved hemocyte-enriched molecular repertoires, which might be implicated in core hemocyte function by cross-species meta-analysis of microarray expression data from Anopheles gambiae and Drosophila melanogaster.
血细胞是蚊子免疫机制的重要组成部分，如吞噬作用、黑化作用和抗菌肽的产生。然而，我们对血细胞特异性分子过程及其对宿主免疫反应的影响的理解仍然有限。为了更好地了解血细胞特有的免疫生理特征，我们对富含血细胞的转录本进行了全基因组分析，并研究了组织中富含的表达模式如何随宿主的免疫状态而变化。我们的微阵列数据表明，富含血细胞的转录组是动态的和依赖于上下文的。分析在循环血细胞中因细菌挑战而富集的转录本相对于尸体增加了一个维度，以评估感染反应基因和免疫相关基因家族。我们从其他可能与免疫反应组织共享的组织中解析了血细胞特有的转录变化模式，并确定了在血细胞中优先诱导的基因簇，这可能反映了它们在细胞类型特异性功能中的参与。此外，该研究还揭示了保守的富含血细胞的分子库，通过对疟蚊和黑腹果蝇的微阵列表达数据进行跨物种荟萃分析，这些分子库可能与核心血细胞功能有关。
