State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:83-90. doi: 10.1016/j.colsurfb.2012.06.021. Epub 2012 Jun 29.
To improve the bioactivity and cytocompatibility of biomedical titanium dioxide coating, many efforts have been made to modify its surface composition and topography. Meanwhile, CaSiO(3) was commonly investigated as coating material on titanium implants for fast fixation and firm implant-bone attachment due to its demonstrated bioactivity and osteointegration. In this work, gradient TiO(2)/CaSiO(3) coating on titanium was prepared by a two-step procedure, in which porous and nanostructured TiO(2) coating on titanium was prepared by plasma electrolytic oxidation in advance, and then needle and flake-like CaSiO(3) nanocrystals were deposited on the TiO(2) coating surface by electron beam evaporation. In view of the potential clinical applications, apatite-forming ability of the TiO(2)/CaSiO(3) coating was evaluated by simulated body fluid (SBF) immersion tests, and MG63 cells were cultured on the surface of the coating to investigate its cytocompatibility. The results show that deposition of CaSiO(3) significantly enhanced the apatite-forming ability of nanostructured TiO(2) coating in SBF. Meanwhile, the MG63 cells on TiO(2)/CaSiO(3) coating show higher proliferation rate and vitality than that on TiO(2) coating. In conclusion, the porous and nanostructured TiO(2)/CaSiO(3) coating on titanium substrate with good apatite-forming ability and cytocompatibility is a potential candidate for bone tissue engineering and implant coating.
为了提高生物医学二氧化钛涂层的生物活性和细胞相容性，人们已经做出了许多努力来修饰其表面成分和形貌。同时，由于 CaSiO(3)具有生物活性和骨整合性，因此常被研究作为钛植入物的涂层材料，以实现快速固定和牢固的植入物-骨附着。在这项工作中，通过两步程序在钛上制备了梯度 TiO(2)/CaSiO(3)涂层，其中通过等离子体电解氧化预先在钛上制备了多孔和纳米结构的 TiO(2)涂层，然后通过电子束蒸发在 TiO(2)涂层表面沉积了针状和片状 CaSiO(3)纳米晶体。鉴于潜在的临床应用，通过模拟体液（SBF）浸泡试验评估了 TiO(2)/CaSiO(3)涂层的成磷能力，并在涂层表面培养 MG63 细胞以研究其细胞相容性。结果表明，CaSiO(3)的沉积显著增强了 SBF 中纳米结构 TiO(2)涂层的成磷能力。同时，TiO(2)/CaSiO(3)涂层上的 MG63 细胞比 TiO(2)涂层上的细胞具有更高的增殖率和活力。总之，具有良好成磷能力和细胞相容性的多孔和纳米结构 TiO(2)/CaSiO(3)涂层是骨组织工程和植入物涂层的潜在候选材料。
