Ni Siyu, Chang Jiang
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
J Biomater Appl. 2009 Aug;24(2):139-58. doi: 10.1177/0885328208094745. Epub 2008 Sep 18.
CaSiO3 (CS) ceramics have been regarded as a potential bioactive material for bone regeneration. Mg2SiO4 (M2S) ceramic has been reported as a novel bioceramic with higher mechanical properties and good biocompatibility recently. beta-Ca2(PO4)2 (beta-TCP) ceramic is a well-known bioactive and degradable material for bone repair. The aim of this study is to investigate and compare the effect of three bioceramics with different chemical composition on the in vitro degradation, apatite-forming ability in simulated body fluid (SBF) and cytocompatibility. The degradation was evaluated through the activation energy of Si or P ion released from ceramics and the weight loss of the ceramics in Tris-HCl buffer solution. Formation of bone-like apatite on different bioceramic surfaces was investigated in SBF. The presence of bone-like apatite layer on the material surface after soaking in SBF was demonstrated by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM). The effect of ionic products from the three kinds of material dissolution on osteoblast-like cell proliferation was investigated. The results showed that the degradation rate of CS was much faster than that of beta-TCP and M2S ceramics. Apatite formation occurred on the CS ceramics quickly. However, it was less likely to occur on the surfaces of beta-TCP and M2S ceramics. The ionic products from extracts of CS and M2S could stimulate osteoblast-like cell proliferation at certain concentration range throughout the 6-day culture period.
硅酸钙（CS）陶瓷被认为是一种用于骨再生的潜在生物活性材料。据报道，硅酸镁（M2S）陶瓷是一种新型生物陶瓷，具有较高的机械性能和良好的生物相容性。β-磷酸钙（β-TCP）陶瓷是一种众所周知的用于骨修复的生物活性和可降解材料。本研究的目的是研究和比较三种化学成分不同的生物陶瓷对体外降解、在模拟体液（SBF）中形成磷灰石的能力以及细胞相容性的影响。通过从陶瓷中释放的Si或P离子的活化能以及陶瓷在Tris-HCl缓冲溶液中的重量损失来评估降解情况。在SBF中研究了不同生物陶瓷表面骨样磷灰石的形成。通过X射线衍射（XRD）、场发射扫描电子显微镜（FESEM）证实了在SBF中浸泡后材料表面骨样磷灰石层的存在。研究了三种材料溶解产生的离子产物对成骨样细胞增殖的影响。结果表明，CS的降解速率比β-TCP和M2S陶瓷快得多。CS陶瓷上很快就形成了磷灰石。然而，β-TCP和M2S陶瓷表面则不太容易形成。在整个6天的培养期内，CS和M2S提取物中的离子产物在一定浓度范围内可刺激成骨样细胞增殖。
