Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-742, Republic of Korea.
Plant Physiol Biochem. 2012 Sep;58:106-15. doi: 10.1016/j.plaphy.2012.06.015. Epub 2012 Jun 28.
Brassica is an important vegetable group worldwide that is impacted by biotic and abiotic stresses. Molecular biology techniques offer the most efficient approach to address these concerns. Inducible plant defense responses include the production of pathogenesis-related (PR) proteins, and chitinases are very important PR proteins. We collected 30 chitinase like genes, three from our full-length cDNA library of Brassica rapa cv. Osome and 27 from Brassica databases. Sequence analysis and comparison study confirmed that they were all class I-V and VII chitinase genes. These genes also showed a high degree of homology with other biotic stress resistance-related plant chitinases. An organ-specific expression of these genes was observed and among these, seven genes showed significant responses after infection with Fusarium oxysporum f.sp. conglutinans in cabbage and sixteen genes showed responsive expression after abiotic stress treatments in Chinese cabbage. BrCLP1, 8, 10, 17 and 18 responded commonly after biotic and abiotic stress treatments indicating their higher potentials. Taken together, the results presented herein suggest that these chitinase genes may be useful resources in the development of stress resistant Brassica.
芸薹属是全球范围内一类重要的蔬菜，受到生物和非生物胁迫的影响。分子生物学技术是解决这些问题最有效的方法。诱导植物防御反应包括产生与病程相关的（PR）蛋白，几丁质酶是非常重要的 PR 蛋白。我们收集了 30 个几丁质酶类似基因，其中 3 个来自芸薹属作物白菜的全长 cDNA 文库，27 个来自芸薹属数据库。序列分析和比较研究证实它们都是 I-V 和 VII 类几丁质酶基因。这些基因与其他生物胁迫抗性相关的植物几丁质酶也具有高度同源性。这些基因在组织上具有特异性表达，其中 7 个基因在白菜感染尖孢镰刀菌后表现出显著的响应，16 个基因在白菜受到非生物胁迫处理后表现出响应性表达。BrCLP1、8、10、17 和 18 在生物和非生物胁迫处理后共同响应，表明它们具有更高的潜力。综上所述，本文的研究结果表明，这些几丁质酶基因可能是培育抗胁迫芸薹属作物的有用资源。
