Pereira Wendell Jacinto, Melo Arthur Tavares de Oliveira, Coelho Alexandre Siqueira Guedes, Rodrigues Fabiana Aparecida, Mamidi Sujan, Alencar Sérgio Amorim de, Lanna Anna Cristina, Valdisser Paula Arielle Mendes Ribeiro, Brondani Claudio, Nascimento-Júnior Ivanildo Ramalho do, Borba Tereza Cristina de Oliveira, Vianello Rosana Pereira
Universidade Federal de Goiás, Instituto de Ciências Biológicas, Goiânia, GO, Brazil.
Universidade de Brasília, Departamento de Biologia Celular, Brasília, DF, Brazil.
Genet Mol Biol. 2020 Mar 16;43(1):e20180259. doi: 10.1590/1678-4685-GMB-2018-0259. eCollection 2020.
Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.
通过RNA-Seq对干旱敏感（DS）和耐旱（DT）基因型的叶片和根组织中与干旱胁迫响应相关的基因进行了表征。总共鉴定出代表28590个基因的54750个转录本；其中，1648个是高保真的（12个文库的合并），并且是首次在安第斯种质中描述。在1239个差异表达基因（DEG）中，458个在DT中被鉴定出来，主要是氧化应激、对刺激的反应和激酶活性类别中的基因。与DS（T150）相比，DT（T75）中大多数与根中氧化还原术语相关的基因被早期触发，这表明通过减少ROS的损害来实现耐受的机制。在DT叶片中上调的DEG富集的KEGG中，两个与含硫化合物形成相关的通路在所有处理中都是常见的，含硫化合物以其参与对非生物胁迫的耐受性而闻名。通过qPCR，88.64%的DEG得到了验证。总共鉴定出151283个变体，并对85780个进行了功能效应估计。原始数据文件已提交到NCBI数据库。转录组图谱揭示了干旱条件下的新基因和异构体。这些结果有助于更好地理解豆类的耐旱机制。
