Jung Hee-Jeong, Manoharan Ranjith Kumar, Park Jong-In, Chung Mi-Young, Lee Jeongyeo, Lim Yong-Pyo, Hur Yoonkang, Nou Ill-Sup
Department of Horticulture, Sunchon National University, Suncheon, Jeonnam 540-950, Republic of Korea.
Department of Agricultural Education, Sunchon National University, Suncheon, Jeonnam 540-950, Republic of Korea.
Int J Genomics. 2014;2014:204969. doi: 10.1155/2014/204969. Epub 2014 Dec 31.
The yellow color of inner leaves in Chinese cabbage depends on its lutein and carotene content. To identify responsible genes for yellow pigmentation in leaves, the transcriptome profiles of white (Kenshin) and yellow leaves (Wheessen) were examined using the Br300K oligomeric chip in Chinese cabbage. In yellow leaves, genes involved in carotene synthesis (BrPSY, BrPDS, BrCRTISO, and BrLCYE), lutein, and zeaxanthin synthesis (BrCYP97A3 and BrHYDB) were upregulated, while those associated with carotene degradation (BrNCED3, BrNCED4, and BrNCED6) were downregulated. These expression patterns might support that the content of both lutein and total carotenoid was much higher in the yellow leaves than that in the white leaves. These results indicate that the yellow leaves accumulate high levels of both lutein and β-carotene due to stimulation of synthesis and that the degradation rate is inhibited. A large number of responsible genes as novel genes were specifically expressed in yellow inner leaves, suggesting the possible involvement in pigment synthesis. Finally, we identified three transcription factors (BrA20/AN1-like, BrBIM1, and BrZFP8) that are specifically expressed and confirmed their relatedness in carotenoid synthesis from Arabidopsis plants.
大白菜内层叶片的黄色取决于其叶黄素和胡萝卜素含量。为了鉴定叶片黄色素沉着的相关基因，利用大白菜的Br300K寡聚芯片检测了白色叶片（Kenshin）和黄色叶片（Wheessen）的转录组图谱。在黄色叶片中，参与胡萝卜素合成的基因（BrPSY、BrPDS、BrCRTISO和BrLCYE）、叶黄素和玉米黄质合成的基因（BrCYP97A3和BrHYDB）上调，而与胡萝卜素降解相关的基因（BrNCED3、BrNCED4和BrNCED6）下调。这些表达模式可能支持黄色叶片中叶黄素和总类胡萝卜素的含量远高于白色叶片。这些结果表明，黄色叶片由于合成受到刺激而积累了高水平的叶黄素和β-胡萝卜素，并且降解速率受到抑制。大量作为新基因的相关基因在黄色内层叶片中特异性表达，表明它们可能参与色素合成。最后，我们鉴定了三个特异性表达的转录因子（BrA20/AN1-like、BrBIM1和BrZFP8），并证实了它们与拟南芥植物类胡萝卜素合成的相关性。
