将RP4和R68.45因子转移至根瘤菌。
Transfer of RP4 and R68.45 factors to Rhizobium.
作者信息
Kowalczuk E, Lorkiewicz Z
出版信息
Acta Microbiol Pol. 1979;28(3):221-9.
PMID:92171
Abstract
Two R factor were introduced by conjugation into Rhizobium trifolii and Rhizobium meliloti strains at a frequency of 10(-5) to 10(-6). Plasmids RP4 from Escherichia coli J53 and R68.45 from Pseudomonas aeruginosa PAO.25 were maintained stably in Rhizobium hosts and could be retransferred to other Rhizobium recipients. Some of the transconjugants were able to mobilize chromosome and transfer his or met genes in intra-, and interspecies matings.
摘要
通过接合作用，两种R因子以10⁻⁵至10⁻⁶的频率被导入三叶草根瘤菌和苜蓿根瘤菌菌株中。来自大肠杆菌J53的质粒RP4和来自铜绿假单胞菌PAO.25的质粒R68.45在根瘤菌宿主中稳定维持，并可再次转移至其他根瘤菌受体。一些接合子能够在种内和种间交配中动员染色体并转移组氨酸或甲硫氨酸基因。
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