Department of Breast Surgery, St. George’s Hospital and Medical School, University of London, London, U.K..
Cancer Genomics Proteomics. 2012 Jul-Aug;9(4):199-201.
This pilot study is the first to focus on the potential role of death-associated protein 1 (DAP1) in human breast cancer.
A total of 153 samples were studied. DAP1 transcription levels were determined using quantitative polymerase chain reaction (qPCR). Transcript levels within breast cancer specimens were compared to those of normal background tissues and correlated with clinicopathological data accumulated over a 10-year follow-up period.
The expression of DAP1 mRNA was demonstrated to decrease with increasing Nottingham Prognostic Index (NPI2 vs. NPI3, p=0.0026), and TNM stage (TNM1 vs. 4, p=0.0039). Lower DAP1 expression levels were significantly associated with local recurrence (p=0.02) and distant metastasis (p=0.001).
This study demonstrates an inverse association between DAP1 mRNA levels and tumour stage and clinical outcome in breast cancer; thus, providing evidence that DAP1 plays a pro-apoptotic role in human breast cancer. The relationship between oncogenesis and the autophagy pathway merits further investigation.
本研究为首次聚焦于死亡相关蛋白 1（DAP1）在人乳腺癌中潜在作用的探索性研究。
共研究了 153 例样本。采用实时定量聚合酶链反应（qPCR）检测 DAP1 转录水平。将乳腺癌标本中的转录水平与 10 年随访期内积累的临床病理数据进行比较。
研究表明 DAP1mRNA 的表达随着诺丁汉预后指数（NPI2 与 NPI3，p=0.0026）和 TNM 分期（TNM1 与 4，p=0.0039）的增加而降低。较低的 DAP1 表达水平与局部复发（p=0.02）和远处转移（p=0.001）显著相关。
本研究表明 DAP1mRNA 水平与乳腺癌的肿瘤分期和临床结局呈负相关，提示 DAP1 在人乳腺癌中发挥促凋亡作用。致癌作用与自噬途径之间的关系值得进一步研究。
