MS FRCS, London Breast Institute, The Princess Grace Hospital, 45 Nottingham Place, London, W1U 5NY, UK.
Anticancer Res. 2012 Feb;32(2):671-4.
This pilot study is the first to focus on the potential role for death-associated protein 3 (DAP3) in human breast cancer.
A total of 153 samples were studied. The levels of transcription of DAP3 were determined using quantitative polymerase chain reaction (qPCR). Transcript levels within breast cancer specimens were compared to those of normal background tissues and correlated with clinicopathological data accumulated by over a 10-year follow-up period.
The expression of DAP3 mRNA was demonstrated to decrease with increasing Nottingham Prognostic Index (NPI2 vs. 3, p=0.036), TNM stage (TNM1 vs. 3, p=0.07), and tumour grade (grade 1 vs. 3, p=0.08). Lower DAP3 expression levels were significantly associated with local recurrence (p=0.013), distant metastasis (p=0.0057) and mortality (p=0.019).
This study demonstrates an inverse association between DAP3 mRNA levels and tumour stage and clinical outcome in breast cancer, consistent with the pro-apoptosis function of DAP3. Further research is required in order to confirm our findings and clarify the mechanisms that regulate DAP3 expression in human breast cancer.
本研究为首次聚焦于凋亡相关蛋白 3（DAP3）在人乳腺癌中潜在作用的探索性研究。
共研究了 153 个样本。采用实时定量聚合酶链反应（qPCR）法检测 DAP3 的转录水平。比较了乳腺癌标本内的转录水平与经过 10 年以上随访积累的临床病理数据背景正常组织中的转录水平。
DAP3 mRNA 的表达与 Nottingham 预后指数（NPI2 与 3 相比，p=0.036）、TNM 分期（TNM1 与 3 相比，p=0.07）和肿瘤分级（1 级与 3 级相比，p=0.08）呈负相关。DAP3 表达水平较低与局部复发（p=0.013）、远处转移（p=0.0057）和死亡（p=0.019）显著相关。
本研究表明，DAP3 mRNA 水平与乳腺癌的肿瘤分期和临床结局呈负相关，与 DAP3 的促凋亡功能一致。需要进一步研究以证实我们的发现，并阐明调节人乳腺癌中 DAP3 表达的机制。
