University of Science and Technology (UST), 217 Gajungro, Yuseong, Daejeon 305-350, Republic of Korea.
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1329-1334. doi: 10.1099/ijs.0.042796-0. Epub 2012 Jul 13.
A gram-negative, non-spore-forming, non-flagellated, motile-by-gliding rod, designated SSK2-3(T), was isolated from the junction between seawater and a freshwater spring at Jeju island, South Korea. Strain SSK2-3(T) grew optimally at 25-30 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SSK2-3(T) clustered with type strains of species of the genus Mariniflexile, with which it exhibited 97.2-97.8 % 16S rRNA gene sequence similarity. Sequence similarity between the isolate and the other strains used in the phylogenetic analysis was <95.6 %. Strain SSK2-3(T) contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 G and C15 : 0 as the major fatty acids. The major polar lipids of strain SSK2-3(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-3(T) was 32.4 mol%. DNA-DNA relatedness between the isolate and Mariniflexile gromovii KCTC 12570(T), Mariniflexile fucanivorans DSM 18792(T) and Mariniflexile aquimaris HWR-17(T) was 19, 15 and 20 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK2-3(T) is separate from other members of the genus Mariniflexile. On the basis of the data presented, strain SSK2-3(T) is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile jejuense sp. nov. is proposed. The type strain is SSK2-3(T) ( = KCTC 23958(T) = CCUG 62414(T)). An emended description of the genus Mariniflexile is given.
一株革兰氏阴性、非孢子形成、无鞭毛、通过滑行运动的杆状、可运动的菌株 SSK2-3(T)，从韩国济州岛海水和淡水泉交界处分离得到。菌株 SSK2-3(T)在 25-30°C、pH7.0-7.5 和 2%（w/v）NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，菌株 SSK2-3(T)与 Mariniflexile 属的模式种聚在一起，与其他模式种的 16S rRNA 基因序列相似度为 97.2-97.8%。与系统发育分析中使用的其他菌株的序列相似度<95.6%。菌株 SSK2-3(T)含有 MK-6 作为主要的menaquinone，iso-C15:0、iso-C15:1 G 和 C15:0 作为主要的脂肪酸。菌株 SSK2-3(T)的主要极性脂质是磷脂酰乙醇胺和一种未鉴定的脂质。菌株 SSK2-3(T)的 DNA G+C 含量为 32.4 mol%。该分离株与 Mariniflexile gromovii KCTC 12570(T)、Mariniflexile fucanivorans DSM 18792(T)和 Mariniflexile aquimaris HWR-17(T)之间的 DNA-DNA 相关性分别为 19%、15%和 20%。差异表型特性，加上系统发育和遗传上的独特性，表明菌株 SSK2-3(T)与 Mariniflexile 属的其他成员不同。基于所提供的数据，菌株 SSK2-3(T)被认为代表 Mariniflexile 属的一个新种，因此建议将其命名为 Mariniflexile jejuense sp. nov.。模式菌株为 SSK2-3(T)（=KCTC 23958(T)=CCUG 62414(T)）。对 Mariniflexile 属的描述进行了修订。
