Park Sooyeon, Kang Chul-Hyung, Park Ja-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2014 Sep;106(3):555-63. doi: 10.1007/s10482-014-0227-z. Epub 2014 Jul 8.
A Gram-negative, aerobic, non-flagellated and rod-shaped bacterial strain able to move by gliding, designated TYO-10(T), was isolated from an oyster collected at Tongyoung on the South Sea, South Korea. Strain TYO-10(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain TYO-10(T) joined the cluster comprising the type strains of Mariniflexile species. Strain TYO-10(T) exhibited 16S rRNA gene sequence similarity values of 95.4-95.7 % to the type strains of Mariniflexile species and of less than 95.4 % to the type strains of other recognized species. Strain TYO-10(T) was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, anteiso-C15:0, iso-C17:0 3-OH and iso-C15:0 3-OH as the major fatty acids. The major polar lipids of strain TYO-10(T) were identified as phosphatidylethanolamine and an unidentified lipid, which is similar to those of the type strains of Mariniflexile species, but different from those of other phylogenetically related species. The DNA G+C content of strain TYO-10(T) was determined to be 35.9 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain TYO-10(T) is separated from other Mariniflexile species. On the basis of the data presented, strain TYO-10(T) is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile ostreae sp. nov. is proposed. The type strain is TYO-10(T) (= KCTC 42113(T) = CECT 8622(T)).
从韩国南海统营采集的一只牡蛎中分离出一株革兰氏阴性、需氧、无鞭毛、杆状、能够滑行运动的细菌菌株，命名为TYO-10(T)。发现菌株TYO-10(T)在30℃、pH 7.0 - 8.0以及2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树显示，菌株TYO-10(T)归入包含Mariniflexile属模式菌株的簇。菌株TYO-10(T)与Mariniflexile属模式菌株的16S rRNA基因序列相似性值为95.4 - 95.7%，与其他已确认属的模式菌株的相似性值小于95.4%。发现菌株TYO-10(T)以MK-6作为主要甲基萘醌，以异C15:0、异C15:1 G、anteiso-C15:0、异C17:0 3-OH和异C15:0 3-OH作为主要脂肪酸。菌株TYO-10(T)的主要极性脂被鉴定为磷脂酰乙醇胺和一种未鉴定的脂类，这与Mariniflexile属模式菌株的相似，但与其他系统发育相关属的不同。菌株TYO-10(T)的DNA G+C含量测定为35.9 mol%。其不同的表型特征以及系统发育独特性表明，菌株TYO-10(T)与其他Mariniflexile属物种不同。根据所提供的数据，菌株TYO-10(T)被认为代表Mariniflexile属的一个新物种，为此提议命名为Mariniflexile ostreae sp. nov.。模式菌株为TYO-10(T)（= KCTC 42113(T) = CECT 8622(T)）。
