Schick J, Doerfler W
J Gen Virol. 1979 Apr;43(1):217-22. doi: 10.1099/0022-1317-43-1-217.
Temperature-sensitive (ts) mutants of adenovirus type 2 (Ad2), which are deficient in virus DNA synthesis at the non-permissive temperature, have been used to investigate whether virus DNA replication is required for the occurrence of high mol. wt. Ad2 DNA (greater than 100S, 50 to 90S) in human cells productively infected with Ad2. The high mol. wt. virus DNA has been previously shown to consist of virus and cellular DNA molecules covalently linked. The present data indicate that after infection with DNA-ts mutants, the production of high mol.wt. virus DNA is much less sensitive to restrictive conditions than the synthesis of unit length (34S) Ad2 DNA. This finding lends further support to the idea that the occurrence of high mol. wt. virus DNA is independent of the synthesis of unit length virus DNA.
2型腺病毒（Ad2）的温度敏感（ts）突变体在非允许温度下病毒DNA合成存在缺陷，已被用于研究在被Ad2有效感染的人类细胞中，病毒DNA复制对于高分子量Ad2 DNA（大于100S，50至90S）的出现是否是必需的。先前已表明高分子量病毒DNA由共价连接的病毒和细胞DNA分子组成。目前的数据表明，在用DNA-ts突变体感染后，高分子量病毒DNA的产生对限制条件的敏感性远低于单位长度（34S）Ad2 DNA的合成。这一发现进一步支持了高分子量病毒DNA的出现独立于单位长度病毒DNA合成的观点。
