Ledinko N
J Gen Virol. 1979 Mar;42(3):651-4. doi: 10.1099/0022-1317-42-3-651.
The highly oncogenic human adenovirus type 12 temperature sensitive mutant. H12ts401, is unable to maintain the growth characteristics of transformed cells at the non-permissive temperature. In lytic infection, the 401 gene function is required to produce virus DNA. In the present study, virus DNA synthesized in ts401-infected human cells after temperature shift-up was characterized. No apparent suppression of DNA chain elongation or ligation occurs at the non-permissive temperature, but, as shown by density labelling, new initiation of virus DNA replication is inhibited under this condition. The results indicate that the 401 gene function is involved in the initiation of virus DNA synthesis in the lytic cycle.
高致癌性的人12型腺病毒温度敏感突变体H12ts401在非允许温度下无法维持转化细胞的生长特性。在裂解感染中，401基因功能是产生病毒DNA所必需的。在本研究中，对ts401感染的人细胞在温度升高后合成的病毒DNA进行了表征。在非允许温度下，DNA链的延伸或连接没有明显受到抑制，但是，如密度标记所示，在这种条件下病毒DNA复制的新起始受到抑制。结果表明，401基因功能参与了裂解周期中病毒DNA合成的起始过程。
