使用快速高效液相色谱法检测军团菌属的马尿酸盐水解情况。
Detection of hippurate hydrolysis by Legionella spp. by using a rapid high-performance liquid chromatography method.
作者信息
Marmet D, Bornstein N, Fleurette J
机构信息
Laboratoire National de la Santé, Centre National de Référence des Légionelloses, Faculté de Médecine, Lyon, France.
出版信息
J Clin Microbiol. 1990 Dec;28(12):2828-9. doi: 10.1128/jcm.28.12.2828-2829.1990.
Abstract
A rapid high-performance liquid chromatography method was developed to determine hippurate hydrolysis by Legionella spp. Benzoic acid, an end product of enzymatic activity, was directly detected by high-performance liquid chromatography after 1 and 24 h of incubation in 1% sodium hippurate. Because of its sensitivity, this procedure offers more precise identification of some Legionella spp.
摘要
开发了一种快速高效液相色谱法来测定军团菌属对马尿酸盐的水解作用。苯甲酸是酶活性的终产物，在1%马尿酸钠中孵育1小时和24小时后，通过高效液相色谱法直接检测苯甲酸。由于其灵敏度高，该方法能更精确地鉴定某些军团菌属。
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