从一名致命肺炎患者身上分离出的嗜肺军团菌血清型兰辛3，以及嗜肺军团菌嗜肺亚种新亚种、嗜肺军团菌弗雷泽亚种新亚种和嗜肺军团菌帕斯库莱亚种新亚种的描述
Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov.
作者信息
Brenner D J, Steigerwalt A G, Epple P, Bibb W F, McKinney R M, Starnes R W, Colville J M, Selander R K, Edelstein P H, Moss C W
机构信息
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1988 Sep;26(9):1695-703. doi: 10.1128/jcm.26.9.1695-1703.1988.
Previous DNA relatedness and enzyme electrophoretic mobility studies indicated heterogeneity among strains of Legionella pneumophila serogroups 1, 4, 5, and Lansing 3 (a new, as yet unnumbered serogroup). In this study 60 L. pneumophila strains were studied by DNA hybridization (hydroxyapatite method) to assess their genomic relatedness. These strains were also studied biochemically and serologically to determine whether they formed one or more phenotypic groups. DNA relatedness studies identified three groups. DNA group 1 contained the type strain Philadelphia 1 and strains from serogroups 1 through 14 of L. pneumophila. The average relatedness of DNA group 1 strains was 88% at 60 degrees C with 1.1% divergence in related sequences and 85% at 75 degrees C. DNA group 2 contained strain Los Angeles 1, the reference strain of serogroup 4, and strains of serogroups 1, 4, 5, and Lansing 3, an unnumbered serogroup. Average relatedness of DNA group 2 strains was 84% at 60 degrees C with 0.7% divergence and 87% at 75 degrees C. Reciprocal relatedness of DNA groups 1 and 2 was approximately 67% at 60 degrees C with 6.0% divergence and 48% at 75 degrees C. DNA group 3 strains were in serogroup 5. They were 98% related at 60 degrees C with 0.5% divergence and 97% related at 75 degrees C. Reciprocal relatedness of DNA group 3 and DNA group 1 was approximately 74% at 60 degrees C with 5.3% divergence and 43% at 75 degrees C, and reciprocal relatedness of DNA groups 3 and 2 was 66% at 60 degrees C with 5.7% divergence and 55% at 75 degrees C. The DNA groups could not be separated biochemically or serologically or by cell wall fatty acid and isoprenoid quinone composition. Three subspecies of L. pneumophila are proposed to accommodate the three DNA groups: L. pneumophila subsp. pneumophila subsp. nov. for DNA group 1, L. pneumophila subsp. fraseri subsp. nov. for DNA group 2, and pneumophila subsp. pascullei subsp. nov. for DNA group 3.
先前有关DNA相关性和酶电泳迁移率的研究表明，嗜肺军团菌血清群1、4、5和兰辛3型（一个新的、尚未编号的血清群）的菌株之间存在异质性。在本研究中，通过DNA杂交（羟基磷灰石法）对60株嗜肺军团菌菌株进行了研究，以评估它们的基因组相关性。还对这些菌株进行了生化和血清学研究，以确定它们是否形成一个或多个表型组。DNA相关性研究确定了三个组。DNA组1包含模式菌株费城1型以及嗜肺军团菌血清群1至14的菌株。DNA组1菌株在60℃时的平均相关性为88%，相关序列的差异为1.1%，在75℃时为85%。DNA组2包含洛杉矶1型菌株，血清群4的参考菌株，以及血清群1、4、5和兰辛3型（一个未编号的血清群）的菌株。DNA组2菌株在60℃时的平均相关性为84%，差异为0.7%，在75℃时为87%。DNA组1和DNA组2在60℃时的相互相关性约为67%，差异为6.0%，在75℃时为48%。DNA组3菌株属于血清群5。它们在60℃时的相关性为98%，差异为0.5%，在75℃时为97%。DNA组3与DNA组1在60℃时的相互相关性约为74%，差异为5.3%，在75℃时为43%，DNA组3与DNA组2在60℃时的相互相关性为66%，差异为5.7%，在75℃时为55%。这些DNA组不能通过生化、血清学方法或根据细胞壁脂肪酸和类异戊二烯醌组成来区分。现提出嗜肺军团菌的三个亚种以容纳这三个DNA组：DNA组1对应的嗜肺军团菌嗜肺亚种新亚种，DNA组2对应的嗜肺军团菌弗雷泽亚种新亚种，以及DNA组3对应的嗜肺军团菌帕斯库莱亚种新亚种。
Zotero 插件