用于检测B族链球菌水解马尿酸盐的快速比色试验。
Rapid, colorimetric test for the determination of hippurate hydrolysis by group B Streptococcus.
作者信息
Edberg S C, Samuels S
出版信息
J Clin Microbiol. 1976 Jan;3(1):49-50. doi: 10.1128/jcm.3.1.49-50.1976.
Abstract
A colorimetric test for the determination of hippurate hydrolysis was developed. Brain heart infusion broth made with 1% sodium hippurate served as the test medium. Hydrolysis was determined by the addition of two chemical developers, M (rhodamine B) and A (uranium acetate). A dark pink color indicated hydrolysis; no color change indicated no hydrolysis. The method was efficacious in either rapid or overnight incubation. One hundred twenty-five strains of group B, 44 strains of group A, 15 strains of group C, and 10 strains of group G Streptococcus were tested. By using the Lancefield method as the standard, there was 100% agreement with both the colorimetric and ferric chloride tests for hippurate hydrolysis, and 96% agreement with the CAMP test.
摘要
开发了一种用于测定马尿酸盐水解的比色试验。用1%马尿酸钠制成的脑心浸液肉汤用作试验培养基。通过添加两种化学显色剂M（罗丹明B）和A（醋酸铀）来测定水解情况。深粉色表示水解；无颜色变化表示未水解。该方法在快速培养或过夜培养中均有效。对125株B组、44株A组、15株C组和10株G组链球菌进行了测试。以兰斯菲尔德法为标准，在马尿酸盐水解的比色试验和氯化铁试验中一致性为100%，在CAMP试验中的一致性为96%。
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