Hilario E, Romero I, Celis H
Departamento de Bioenergética, Instituto de Fisiología Celular, UNAM, México D.F.
J Biochem Biophys Methods. 1990 Sep-Oct;21(3):197-207. doi: 10.1016/0165-022x(90)90013-3.
In this work we report a detailed characterization of the metallochromic Zincon. Zincon forms complexes with Zn2+ and Cu2+, producing change in colour; the complexes with Fe2+, Mn2+ and Ca2+ cause the bleaching of the Zincon solutions. Mg2+ does not interact with Zincon nor does it change its spectral characteristics. The presence of Ca2+ and Mg2+ does not interfere with the spectral characteristics of the Zn-Zincon complex. The Kd, Ks and delta epsilon values for the complexes were determined. The delta epsilon values were very high, making this spectrophotometric method very sensitive. The complex Zn-Zincon is fully reversible; however, the complex Cu-Zincon is only partially reversible. The free Zincon, and the complexes Zn-Zincon and Cu-Zincon, does not partition into organic solvents, does not permeate liposome membrane, and neither does it interact with biological membranes. All these characteristics make the metallochromic Zincon useful in biological systems.
在这项工作中，我们报告了对金属变色试剂锌试剂的详细表征。锌试剂与Zn2+和Cu2+形成配合物，导致颜色变化；与Fe2+、Mn2+和Ca2+形成的配合物会使锌试剂溶液褪色。Mg2+不与锌试剂相互作用，也不会改变其光谱特性。Ca2+和Mg2+的存在不会干扰Zn-锌试剂配合物的光谱特性。测定了配合物的解离常数（Kd）、稳定常数（Ks）和摩尔吸光系数差值（δε）。δε值非常高，使得这种分光光度法非常灵敏。Zn-锌试剂配合物是完全可逆的；然而，Cu-锌试剂配合物只是部分可逆。游离的锌试剂以及Zn-锌试剂和Cu-锌试剂配合物都不会分配到有机溶剂中，不会渗透脂质体膜，也不会与生物膜相互作用。所有这些特性使得金属变色试剂锌试剂在生物系统中很有用。
