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[采用酶联免疫吸附测定法检测疑似包虫囊肿患者的抗棘球绦虫IgG抗体]

[Determination of anti-echinococcus IgG antibodies by ELISA in patients with suspected hydatid cyst].

作者信息

Aydın Merve, Adıyaman Gülcan, Doğruman-Al Funda, Kuştimur Semra, Ozkan Seçil

机构信息

Erzincan Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Erzincan, Türkiye.

出版信息

Turkiye Parazitol Derg. 2012;36(2):61-4. doi: 10.5152/tpd.2012.16.

Abstract

OBJECTIVE

Cystic echinococcosis (hydatid cyst) is one of the most important parasitic zoonoses that affect both humans and animals and has been known since prehistoric times. The cystic echinoccosis is a major health problem in our country as well as in many countries worldwide, and serological methods, in addition to imaging techniques, are used in the diagnosis of hydatid cyst. In the present study, anti- Echinococcus antibodies were investigated by ELISA in patient's serum samples, which were routinely delivered to the Medical Microbiology Laboratory of Gazi University Faculty of Medicine due to suspected hydatid cyst.

METHODS

A total of 186 patients with suspected hydatid cysts from several outpatient clinics and departments of Gazi University Hospital were included in the present study. IgG antibodies in serum samples of patients with anti-Echinococcus were investigated by ELISA (Novalisa Echinococcus IgG, NovaTec, Germany).

RESULTS

Anti-Echinococcus IgG seropositivity was determined as 35.5% in patients with suspected hydatid cyst. There are no statistical differences in ELISA positive results relating to gender, adult/child age group and associated clinics.

CONCLUSION

Hydatid cyst seropositivity was higher among patients admitted to our hospital.

摘要

目的

囊型包虫病(包虫囊肿)是影响人类和动物的最重要的寄生虫人畜共患病之一,自史前时代就已为人所知。囊型包虫病在我国以及世界许多国家都是一个主要的健康问题,除了成像技术外,血清学方法也用于包虫囊肿的诊断。在本研究中,通过酶联免疫吸附测定法(ELISA)对因疑似包虫囊肿而常规送往加齐大学医学院医学微生物学实验室的患者血清样本中的抗棘球绦虫抗体进行了研究。

方法

本研究纳入了来自加齐大学医院多个门诊科室的186例疑似包虫囊肿患者。通过ELISA(德国诺华公司的Novalisa棘球绦虫IgG试剂盒)检测患者血清样本中的抗棘球绦虫IgG抗体。

结果

疑似包虫囊肿患者的抗棘球绦虫IgG血清阳性率为35.5%。ELISA阳性结果在性别、成人/儿童年龄组和相关科室方面无统计学差异。

结论

我院收治患者的包虫囊肿血清阳性率较高。

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