Faculty of Science, Department of Analytical Chemistry, Charles University in Prague, Prague, Czech Republic.
Anal Bioanal Chem. 2012 Sep;404(5):1549-54. doi: 10.1007/s00216-012-6242-x. Epub 2012 Jul 17.
The methodology for separations of saccharides in standard electrophoretic systems has been transferred to the short-capillary electrophoresis format. The laboratory-designed apparatus used employs a quartz capillary with an internal diameter of 10 μm, a total length of 10 cm, and an effective length of 4 cm, in combination with contactless conductivity detection. It has been applied to separations of neutral mono- and disaccharides. The saccharides are separated in the anionic form, in solutions of alkali hydroxides, namely, KOH, NaOH, and LiOH. The separation of a model mixture of five saccharides (sucrose, lactose, glucose, fructose, and ribose) takes less than 1 min, the LOD equaling 15, 35, 19, 17, and 24 mg L(-1) and the LOQ equaling 52, 117, 63, 53, and 79 mg L(-1) for sucrose, lactose, glucose, fructose, and ribose, respectively. The technique developed has been used to determine sucrose, glucose and fructose in high-energy drinks. The separation is finished within less than 50 s; the saccharide contents determined are identical with the declared values within the reliability interval in most cases, the RSD value being mostly less than 2%. In general, the separation system developed is very convenient for rapid analyses of large sets of similar samples, e.g., in product quality control or environmental monitoring.
糖的分离方法已从标准电泳系统转移到短毛细管电泳模式。该实验室设计的仪器采用内径为 10μm、总长度为 10cm 且有效长度为 4cm 的石英毛细管，结合非接触式电导检测。该仪器已用于分离中性单糖和二糖。糖以阴离子形式在碱金属氢氧化物溶液（即 KOH、NaOH 和 LiOH）中进行分离。5 种糖（蔗糖、乳糖、葡萄糖、果糖和核糖）的模型混合物的分离不到 1 分钟即可完成，蔗糖、乳糖、葡萄糖、果糖和核糖的检测限分别为 15、35、19、17 和 24mg/L，定量限分别为 52、117、63、53 和 79mg/L。所开发的技术已用于测定高能饮料中的蔗糖、葡萄糖和果糖。分离在不到 50s 内完成；在大多数情况下，所测定的糖含量与声明值在可靠性区间内一致，RSD 值大多小于 2%。总的来说，所开发的分离系统非常适合快速分析大量类似的样品，例如在产品质量控制或环境监测中。
