Institute of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
Anal Chim Acta. 2011 Jul 18;698(1-2):1-5. doi: 10.1016/j.aca.2011.04.055. Epub 2011 May 5.
A capillary electrophoresis (CE) procedure with contactless conductivity detection (C(4)D) has been developed for monitoring of neutral mono- and disaccharides in drinks and foodstuffs. The separation of a mixture of seven neutral saccharides (glucose, fructose, galactose, mannose, ribose, sucrose and lactose) employed a quartz capillary, 5 μm i.d., with an effective length of 18.3 cm, and 75 mM NaOH (pH 12.8) as the background electrolyte (BGE). The limit of detection (LOD) values obtained lied within a range from 0.4 μmol L(-1) for lactose to 0.9 μmol L(-1) for ribose, with a separation time shorter than 140 s. The procedure was successfully applied to determinations of saccharides in fruit juices, Coca-Cola, milk, red and white wines, yoghurts, honey and a foodstuff additive.
已经开发出一种毛细管电泳（CE）程序，带有非接触式电导检测（C（4）D），用于监测饮料和食品中的中性单糖和二糖。使用石英毛细管，内径 5 μm，有效长度 18.3 cm，75 mM NaOH（pH 12.8）作为背景电解质（BGE），分离了七种中性糖（葡萄糖、果糖、半乳糖、甘露糖、核糖、蔗糖和乳糖）的混合物。所得到的检测限（LOD）值范围从乳糖的 0.4 μmol L（-1）到核糖的 0.9 μmol L（-1），分离时间短于 140 s。该程序成功应用于果汁、可口可乐、牛奶、红葡萄酒、白葡萄酒、酸奶、蜂蜜和食品添加剂中糖的测定。
