携带Rms 298的大肠杆菌青霉素酶的生化特性

Biochemical properties of a penicillinase from Escherichia coli carrying Rms 298.

作者信息

Sawada Y, Tai M, Mitsuhashi S

出版信息

Microbiol Immunol. 1977 Oct 20;21(10):545-51. doi: 10.1111/j.1348-0421.1977.tb00322.x.

DOI:10.1111/j.1348-0421.1977.tb00322.x

Abstract

We obtained two R plasmids, i.e., Rms195 and Rms298, from a clinical isolate, E. coli GN5503. Penicillin beta-lactamase (PCase) was extracted from ML1410 Rms195+ and Rms298+, and was purified by chromatography. Rms195 PCase was identical to the type I PCase mediated by R-TEM, RI and Rms212. The isoelectric point of Rms298 PCase was 5.9 and its molecular weight was 21,000 +/- 1,000. The substrate profile and physiochemical properties indicate that Rms298 PCase belongs to the type IV PCase mediated by Rms139 isolated from Pseudomonas aeruginosa.

摘要

我们从临床分离株大肠杆菌GN5503中获得了两个R质粒，即Rms195和Rms298。从携带Rms195+和Rms298+的ML1410中提取青霉素β-内酰胺酶（PCase），并通过色谱法进行纯化。Rms195 PCase与由R-TEM、RI和Rms212介导的I型PCase相同。Rms298 PCase的等电点为5.9，分子量为21,000±1,000。底物谱和理化性质表明，Rms298 PCase属于由铜绿假单胞菌分离出的Rms139介导的IV型PCase。