一种由大肠杆菌中的R因子介导合成的青霉素酶的纯化及其性质
The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli.
作者信息
Datta N, Richmond M H
出版信息
Biochem J. 1966 Jan;98(1):204-9. doi: 10.1042/bj0980204.
Abstract
- The penicillinase (beta-lactamase) from Escherichia coli strain TEM has been purified and its activity against a range of penicillin and cephalosporin derivatives measured. 2. The enzyme shows little resemblance to penicillinases from Bacillus cereus, Bacillus licheniformis and Staphylococcus aureus. 3. The molecular weight of the enzyme is 16700+/-5%, which is about half the value obtained for other penicillinases. 4. The enzyme is most similar in properties to a crude preparation of a penicillinase from Klebsiella (Aerobacter) aerogenes, but clearly different from crude enzyme preparations from other strains of E. coli. 5. Since penicillinase synthesis in E. coli strain TEM is mediated by an R-factor known to infect many other species of Enterobacteriaceae, the appearance of similar enzymes in other Gramnegative species is not surprising.
摘要
- 已对来自大肠杆菌TEM菌株的青霉素酶（β-内酰胺酶）进行了纯化，并测定了其对一系列青霉素和头孢菌素衍生物的活性。2. 该酶与蜡状芽孢杆菌、地衣芽孢杆菌和金黄色葡萄球菌的青霉素酶几乎没有相似之处。3. 该酶的分子量为16700±5%，约为其他青霉素酶所得值的一半。4. 该酶在性质上与产气克雷伯菌（产气气杆菌）青霉素酶的粗制品最为相似，但与其他大肠杆菌菌株的粗酶制品明显不同。5. 由于大肠杆菌TEM菌株中青霉素酶的合成是由一种已知可感染许多其他肠杆菌科物种的R因子介导的，因此在其他革兰氏阴性物种中出现类似的酶并不奇怪。
相似文献
1
The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli.一种由大肠杆菌中的R因子介导合成的青霉素酶的纯化及其性质
Biochem J. 1966 Jan;98(1):204-9. doi: 10.1042/bj0980204.
2
PURIFICATION AND PROPERTIES OF PENICILLINASES FROM TWO STRAINS OF BACILLUS LICHENIFORMIS: A CHEMICAL, PHYSICOCHEMICAL AND PHYSIOLOGICAL COMPARISON.两株地衣芽孢杆菌青霉素酶的纯化及性质：化学、物理化学和生理学比较
Biochem J. 1965 Mar;94(3):666-75. doi: 10.1042/bj0940666.
3
Some relationships between R-factor and chromosomal -lactamase in Gram-negative bacteria.革兰氏阴性菌中R因子与染色体β-内酰胺酶之间的一些关系。
Biochem J. 1971 Jul;123(4):507-12. doi: 10.1042/bj1230507.
4
Comparison of the substrate specificities of the -lactamases from Klebsiella aerogenes 1082E and Enterobacter cloacae P99.产气克雷伯菌1082E和阴沟肠杆菌P99的β-内酰胺酶底物特异性比较。
Appl Microbiol. 1972 Apr;23(4):765-9. doi: 10.1128/am.23.4.765-769.1972.
5
Resistance of Escherichia coli to penicillins. VII. Purification and characterization of a penicillinase mediated by the R factor R1.大肠杆菌对青霉素的抗性。VII. 由R因子R1介导的一种青霉素酶的纯化及特性分析。
J Bacteriol. 1970 Jan;101(1):232-9. doi: 10.1128/jb.101.1.232-239.1970.
6
[Penicillinase-resistant penicillins and cephalosporin, an advance in the battle against resistant microbes].
Dtsch Med Wochenschr. 1977 Aug 26;102(34):1207-10. doi: 10.1055/s-0028-1105480.
7
Correlation between hydrolysis of the -lactam bond of the cephalosporin nucleus and expulsion of the 3-substituent.头孢菌素核β-内酰胺键的水解与3-取代基的脱去之间的相关性。
J Bacteriol. 1972 Jun;110(3):988-91. doi: 10.1128/jb.110.3.988-991.1972.
8
Biochemical properties of a penicillinase from Escherichia coli carrying Rms 298.携带Rms 298的大肠杆菌青霉素酶的生化特性
Microbiol Immunol. 1977 Oct 20;21(10):545-51. doi: 10.1111/j.1348-0421.1977.tb00322.x.
9
Penicillin-binding proteins of Escherichia coli. Comparison of a strain carrying an R-factor and the parent strain.大肠杆菌的青霉素结合蛋白。携带R因子的菌株与亲本菌株的比较。
Biochim Biophys Acta. 1977 Mar 28;491(1):223-31. doi: 10.1016/0005-2795(77)90058-7.
10
Variant of penicillinase mediated by an R factor in Escherichia coli.由大肠杆菌中一种R因子介导的青霉素酶变体。
J Bacteriol. 1970 Nov;104(2):620-9. doi: 10.1128/jb.104.2.620-629.1970.
引用本文的文献
1
Allosteric inhibition of TEM-1 β lactamase: Microsecond molecular dynamics simulations provide mechanistic insights.变构抑制 TEM-1 β-内酰胺酶：微秒分子动力学模拟提供的机制见解。
Protein Sci. 2023 Apr;32(4):e4622. doi: 10.1002/pro.4622.
2
Past and Present Perspectives on β-Lactamases.β-内酰胺酶的过去与现在观点。
Antimicrob Agents Chemother. 2018 Sep 24;62(10). doi: 10.1128/AAC.01076-18. Print 2018 Oct.
3
Molecular epidemiology of clinically significant antibiotic resistance genes.具有临床意义的抗生素耐药基因的分子流行病学
Br J Pharmacol. 2008 Mar;153 Suppl 1(Suppl 1):S406-13. doi: 10.1038/sj.bjp.0707632.
4
Enzymatic Adenylylation of Streptomycin and Spectinomycin by R-Factor-Resistant Escherichia coli.R 因子抗性大肠杆菌对链霉素和壮观霉素的酶腺苷酰化作用。
Infect Immun. 1970 Jan;1(1):109-19. doi: 10.1128/iai.1.1.109-119.1970.
5
R-mediated beta-lactamases and episomal resistance to the beta-lactam drugs in different bacterial hosts.R介导的β-内酰胺酶与不同细菌宿主中β-内酰胺类药物的附加体耐药性。
Antimicrob Agents Chemother. 1974 Jul;6(1):60-72. doi: 10.1128/AAC.6.1.60.
6
Effects of inoculum and beta-lactamase activity in AmpC- and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae clinical isolates tested by using NCCLS ESBL methodology.采用美国国家临床实验室标准委员会（NCCLS）的超广谱β-内酰胺酶（ESBL）检测方法，对产AmpC酶和ESBL的大肠埃希菌及肺炎克雷伯菌临床分离株，检测接种量和β-内酰胺酶活性的影响。
J Clin Microbiol. 2004 Jan;42(1):269-75. doi: 10.1128/JCM.42.1.269-275.2004.
7
A functional classification scheme for beta-lactamases and its correlation with molecular structure.β-内酰胺酶的功能分类方案及其与分子结构的相关性。
Antimicrob Agents Chemother. 1995 Jun;39(6):1211-33. doi: 10.1128/AAC.39.6.1211.
8
Quantitative correlation between penicillin resistance and beta-lactamase activity specified by the R plasmids R1, R1 bla-45, and RP1 in Escherichia coli K-12.大肠杆菌K-12中R质粒R1、R1 bla-45和RP1所赋予的青霉素抗性与β-内酰胺酶活性之间的定量相关性。
Antimicrob Agents Chemother. 1980 Nov;18(5):675-9. doi: 10.1128/AAC.18.5.675.
9
Purification and properties of cephalosporinase in Escherichia coli.大肠杆菌中头孢菌素酶的纯化及性质
Antimicrob Agents Chemother. 1980 Jul;18(1):77-80. doi: 10.1128/AAC.18.1.77.
10
Principal beta-lactamases responsible for resistance to beta-lactam antibiotics in urinary tract infections.导致尿路感染对β-内酰胺类抗生素耐药的主要β-内酰胺酶。
Antimicrob Agents Chemother. 1980 Jun;17(6):929-36. doi: 10.1128/AAC.17.6.929.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Staphylococcal penicillinase and the new penicillins.葡萄球菌青霉素酶与新型青霉素
Biochem J. 1962 May;83(2):229-35. doi: 10.1042/bj0830229.
3
Micro-iodometric assay for penicillinase.青霉素酶的微量碘量法测定
Biochem J. 1962 May;83(2):236-40. doi: 10.1042/bj0830236.
4
WILD-TYPE VARIANTS OF EXOPENICILLINASE FROM STAPHYLOCOCCUS AUREUS.金黄色葡萄球菌外青霉素酶的野生型变体
Biochem J. 1965 Mar;94(3):584-93. doi: 10.1042/bj0940584.
5
PURIFICATION AND PROPERTIES OF PENICILLINASES FROM TWO STRAINS OF BACILLUS LICHENIFORMIS: A CHEMICAL, PHYSICOCHEMICAL AND PHYSIOLOGICAL COMPARISON.两株地衣芽孢杆菌青霉素酶的纯化及性质：化学、物理化学和生理学比较
Biochem J. 1965 Mar;94(3):666-75. doi: 10.1042/bj0940666.
6
STIMULATING AND INHIBITING ANTIBODIES FOR BACTERIAL PENICILLINASE.针对细菌青霉素酶的刺激抗体和抑制抗体
Immunology. 1964 Nov;7(6):707-23.
7
EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.稀溶液的平衡超速离心法
Biochemistry. 1964 Mar;3:297-317. doi: 10.1021/bi00891a003.
8
PURIFICATION AND PROPERTIES OF THE EXOPENICILLINASE FROM STAPHYLOCOCCUS AUREUS.金黄色葡萄球菌外青霉素酶的纯化及性质
Biochem J. 1963 Sep;88(3):452-9. doi: 10.1042/bj0880452.
9
Applications of radioactively labeled marker proteins in density gradient ultracentrifugation.放射性标记的标记蛋白在密度梯度超速离心中的应用。
Anal Biochem. 1963 Mar;5:226-45. doi: 10.1016/0003-2697(63)90120-9.
10
Penicillinases from gram-positive and gram-negative bacteria: a thermodynamic difference.
Nature. 1963 Feb 23;197:769-70. doi: 10.1038/197769a0.
Zotero 插件