Cai Kaigui, Luo Ying, Li Lixiang, Liu Yuehui
Department of Otorhinolaryngology-Head and Neck Surgery, People's Hospital of Guangzhou Huadu District, Guangzhou 510800, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 May;26(9):425-8.
To investigate the expression of MCM2, Ki-67 and Rb and its biological characteristic in human laryngeal squamous cell carcinomas(LSCC).
The expression of MCM2 protein and Rb protein were detected in 60 cases of LSCC, 10 cases of precarcinoma, 10 cases of vocal cord polyps and 10 cases of normal laryngeal tissues, and Ki-67 protein were detected in 60 cases of LSCC and 10 cases of normal laryngeal tissues by Elivision plus immunohistochemical staining, and analyze their relations with clinicopathological characteristics.
The positive expression rate of MCM2 in LSCC was significantly higher than that in precarcinoma and normal laryngeal tissues (P < 0.05), and was positively correlated with pathological grades, clinical stages and lymph node metastases (P < 0.05) of LSCC. The positive expression rate of Rb protein in LSCC was significantly lower than that in precarcinoma and normal laryngeal tissues (P < 0.05). The expression level of MCM2 in LSCC was negatively corelated with Rb (r = -0.542, P < 0.05), the expression level of Ki-67 in LSCC (76.67%) was significantly higher than that in normal laryngeal tissues (30.00%) (P < 0.01) and the expression level of MCM2 in LSCC was positively corelated with Ki-67(r = 0.596, P < 0.01). The LI of MCM2 in the 3-year survival rate of LSCC was significantly lower than that in Ki-67 (P < 0.05).
Over expression of MCM2 and loss of Rb protein were related to the carcinogenesis and development of LSCC. The determination of MCM2 can be an index for estimating the level of malignancy and prognosis of LSCC.
探讨微小染色体维持蛋白2（MCM2）、Ki-67和视网膜母细胞瘤蛋白（Rb）在人喉鳞状细胞癌（LSCC）中的表达及其生物学特性。
采用Elivision plus免疫组织化学染色法检测60例LSCC、10例癌前病变、10例声带息肉及10例正常喉组织中MCM2蛋白和Rb蛋白的表达，检测60例LSCC及10例正常喉组织中Ki-67蛋白的表达，并分析其与临床病理特征的关系。
LSCC中MCM2的阳性表达率显著高于癌前病变及正常喉组织（P<0.05），且与LSCC的病理分级、临床分期及淋巴结转移呈正相关（P<0.05）。LSCC中Rb蛋白的阳性表达率显著低于癌前病变及正常喉组织（P<0.05）。LSCC中MCM2的表达水平与Rb呈负相关（r=-0.542，P<0.05），LSCC中Ki-67的表达水平（76.67%）显著高于正常喉组织（30.00%）（P<0.01），且MCM2在LSCC中的表达水平与Ki-67呈正相关（r=0.596，P<0.01）。LSCC患者3年生存率中MCM2的标记指数显著低于Ki-67（P<0.05）。
MCM2的过表达及Rb蛋白的缺失与LSCC的发生发展有关。MCM2的检测可作为评估LSCC恶性程度及预后的指标。
