Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Curr Mol Med. 2012 Sep;12(8):1028-39. doi: 10.2174/156652412802480862.
Monocytes/macrophages play crucial roles in immunity to microorganisms and are one of the important targets for human immunodeficiency virus (HIV) infection. The phenotypes and function of monocytes in HIV-infected patients were poorly determined. We herein detected the expression of Th1/Th2 cytokine receptors on monocyte subsets in the untreated HIV-infected patients of either long term nonprogressor (LTNP) or chronic infection (CHI). CD14(+)CD16(-) monocytes were significantly increased and CD14(+)CD16(+) monocytes were reduced in patients of LTNP or CHI compared with healthy control. IL-6R expression on CD14(+)CD16(-) monocytes were decreased in patients of LTNP or CHI, whereas IL-4R and IL-10R expression on both CD14(+)CD16(-) and CD14(+)CD16(+) monocyte subsets were increased in patients with LTNP or CHI, as determined by flow cytometry and real time PCR assays. The decreased IL-6R expression and enhanced IL-4R and IL-10R expression were also observed on CD4(+) T cells of these patients, indicating that these changes in monocytes are not cell-specific. CD14(+)CD16(-) monocytes of HIV-infected patients produced less TNF-α and IL-1β but identical levels of IL-6, and IL-12 as the control after IFN-γ/LPS stimulation. However, in the presence of IL-4 or IL10, CD14(+)CD16(-) monocytes of HIV-infected patients produced more TNF-α, IL-6, IL-12 or Il-1β after IFN-γ/LPS stimulation than the healthy control, supporting the impaired IL-4R and IL-10R signal pathways in patients with LTNP and CHI. Therefore, our present study offered the basic information for the Th1/Th2 cytokine receptor expression and function on monocyte subsets in untreated HIV-infected individuals.
单核细胞/巨噬细胞在微生物免疫中发挥关键作用，是人类免疫缺陷病毒（HIV）感染的重要靶点之一。HIV 感染者单核细胞的表型和功能尚未完全确定。本研究检测了未经治疗的长程非进展者（LTNP）或慢性感染者（CHI）HIV 感染者单核细胞亚群中 Th1/Th2 细胞因子受体的表达。与健康对照组相比，LTNP 或 CHI 患者的 CD14(+)CD16(-)单核细胞明显增加，CD14(+)CD16(+)单核细胞减少。LTNP 或 CHI 患者 CD14(+)CD16(-)单核细胞上的 IL-6R 表达减少，而 LTNP 或 CHI 患者 CD14(+)CD16(-)和 CD14(+)CD16(+)单核细胞亚群上的 IL-4R 和 IL-10R 表达增加，流式细胞术和实时 PCR 检测结果表明。这些患者的 CD4(+)T 细胞也观察到 IL-6R 表达减少，IL-4R 和 IL-10R 表达增强，表明这些变化不是细胞特异性的。IFN-γ/LPS 刺激后，与对照组相比，HIV 感染者的 CD14(+)CD16(-)单核细胞产生的 TNF-α 和 IL-1β 减少，但产生的 IL-6 和 IL-12 水平相同。然而，在存在 IL-4 或 IL10 的情况下，HIV 感染者的 CD14(+)CD16(-)单核细胞在 IFN-γ/LPS 刺激后产生的 TNF-α、IL-6、IL-12 或 Il-1β 多于健康对照组，支持 LTNP 和 CHI 患者中 IL-4R 和 IL-10R 信号通路受损。因此，本研究为未治疗的 HIV 感染者单核细胞亚群中 Th1/Th2 细胞因子受体表达和功能提供了基础信息。
