Departments of Allergology and Internal Medicine, Medical University of Bialystok, Bialystok, Poland.
Int Arch Allergy Immunol. 2013;161(2):131-41. doi: 10.1159/000343687. Epub 2013 Jan 23.
Despite widely acknowledged contributions of innate and adaptive immune systems to the pathogenesis of allergic diseases, mutual interactions occurring in vivo between components of those two systems have not been studied in sufficient detail. Here, we wished to investigate whether phenotypic features of monocytes and CD4+ T cells in allergic patients are reciprocally related. Therefore, we recruited 50 untreated house dust mite-sensitive allergic rhinitis patients and 29 non-atopic healthy individuals and performed comprehensive simultaneous flow cytometric analysis of mutual correlations between levels of CD14, CD16, CD163, CD206, CD124 (IL-4R), CD210 (IL-10R) and CD25, CD124, CD127 (IL-7R), CD210, ICOS expression on monocytes and CD4+ T cells, respectively. We found that CD163 monocyte expression in allergic but not healthy subjects is positively correlated with monocyte IL-10R, and, to a lesser extent, CD206, but not IL-4R expression. Levels of CD163 expression were not related to frequencies of CD14++CD16-, CD14++CD16+, and CD14+CD16++ monocyte subsets. In contrast to healthy controls, intensities of monocyte IL-10R in allergic individuals were significantly correlated with monocyte CD206 and IL-4R expression. In addition, levels of monocyte IL-4R and IL-10R monocyte expression were positively correlated to expression of IL-4R and IL-10R on CD4+ T cells in both groups of studied subjects. Interestingly, we demonstrated a significant positive correlation between levels of monocyte CD206 expression and levels of IL-10R and IL-4R expression on CD4+ T cells in allergic but not healthy individuals. In summary, we conclude that allergic rhinitis is associated with a number of phenotypic alterations of circulating monocytes and CD4+ T cells.
尽管先天和适应性免疫系统对过敏性疾病的发病机制有广泛的贡献，但这两个系统的组成部分在体内相互作用的情况尚未得到充分研究。在这里，我们希望研究过敏性患者的单核细胞和 CD4+T 细胞的表型特征是否相互关联。因此，我们招募了 50 名未经治疗的屋尘螨过敏的鼻炎患者和 29 名非过敏的健康个体，并对相互之间单核细胞和 CD4+T 细胞上的 CD14、CD16、CD163、CD206、CD124（IL-4R）、CD210（IL-10R）和 CD25、CD124、CD127（IL-7R）、CD210、ICOS 的表达水平进行了全面的同步流式细胞术分析。我们发现，只有在过敏性患者中，单核细胞 CD163 的表达与单核细胞 IL-10R 呈正相关，与 CD206 呈较弱的正相关，但与 IL-4R 无关。CD163 表达水平与 CD14++CD16-、CD14++CD16+和 CD14+CD16++单核细胞亚群的频率无关。与健康对照组相比，过敏性个体单核细胞 IL-10R 的强度与单核细胞 CD206 和 IL-4R 的表达显著相关。此外，两组研究对象的单核细胞 IL-4R 和 IL-10R 表达水平与 CD4+T 细胞上的 IL-4R 和 IL-10R 表达水平呈正相关。有趣的是，我们发现在过敏性患者中，单核细胞 CD206 表达水平与 CD4+T 细胞上的 IL-10R 和 IL-4R 表达水平呈显著正相关，但在健康对照组中则无此关联。总之，我们的结论是，过敏性鼻炎与循环单核细胞和 CD4+T 细胞的许多表型改变有关。
