Nasr-Esfahani M H, Deemeh M R, Tavalaee M
Department of Reproduction and Development, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Int J Androl. 2012 Aug;35(4):475-84. doi: 10.1111/j.1365-2605.2011.01227.x. Epub 2011 Nov 15.
Spermatozoa contribute to approximately half of the genome of future progeny, and therefore, have a profound impact on embryo development post-fertilization. Sperm selection based on viability and normal morphology does not eliminate the chance for DNA damaged spermatozoa to be inseminated and may account for a considerable percentage of failed embryo development post-ICSI (Intra cytoplasmic sperm injection). Therefore, sperm selection based on functional sperm characteristics to preclude insemination of DNA damaged spermatozoa have paved the way for successful ICSI outcomes. In regard to this, different laboratories have introduced novel procedures to replace traditional or orthodox sperm selection methods. This review attempts to provide information on the scientific bases of each procedure, and pinpoint their advantages and disadvantages. In addition to data from our research, a systematic search on the literature, publications and presentations was carried out using such databases as PubMed and ISI-Web.
精子对未来后代的基因组贡献约一半，因此，对受精后的胚胎发育有深远影响。基于活力和正常形态的精子筛选并不能消除DNA受损精子被授精的可能性，这可能是ICSI（卵胞浆内单精子注射）后胚胎发育失败的一个相当大的比例原因。因此，基于功能性精子特征进行精子筛选以排除DNA受损精子的授精，为ICSI的成功结果铺平了道路。关于这一点，不同的实验室引入了新的程序来取代传统或正统的精子筛选方法。本综述试图提供每个程序的科学依据信息，并指出其优缺点。除了我们研究的数据外，还使用PubMed和ISI-Web等数据库对文献、出版物和报告进行了系统检索。
