Biometry and Nutrition Group, Agharkar Research Institute, Pune-411004, India.
Inorg Chem. 2012 Aug 6;51(15):7960-2. doi: 10.1021/ic301244x. Epub 2012 Jul 17.
In Alzheimer's disease, copper binds to amyloid beta (Aβ) peptide and generates oxidative stress. The coordination of histidine (His) residues to Cu(2+) is still uncertain. We studied Cu(2+) binding to Aβ1-16 peptide using the diethyl pyrocarbonate (DEPC) assay and mass spectrometry. Our results show that only one His is involved in Cu(2+) coordination, which is identified as His6 using mass spectral studies. Novel nickel displacement studies have further supported the proposal that the Cu(2+) binding site of Aβ1-16 peptide resembles the ATCUN motif of human serum albumin.
在阿尔茨海默病中，铜与淀粉样β（Aβ）肽结合并产生氧化应激。组氨酸（His）残基与 Cu（2+）的配位仍然不确定。我们使用二乙基焦碳酸酯（DEPC）测定法和质谱法研究了 Cu（2+）与 Aβ1-16 肽的结合。我们的结果表明，只有一个 His 参与了 Cu（2+）的配位，通过质谱研究鉴定为 His6。新的镍置换研究进一步支持了这样的假设，即 Aβ1-16 肽的 Cu（2+）结合位点类似于人血清白蛋白的 ATCUN 基序。
