Department of Microbiology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat 380 009, India.
World J Microbiol Biotechnol. 2012 Mar;28(3):891-9. doi: 10.1007/s11274-011-0886-0. Epub 2011 Sep 23.
A novel Enterobacter cancerogenus MSA2 is a plant growth promoting gamma-proteobacterium that was isolated from the rhizosphere of Jatropha cucas a potentially important biofuel feed stock plant. Based on phenotypic, physiological, biochemical and phylogenetic studies, strain MSA2 could be classified as a member of E. cancerogenus. However, comparisons of characteristics with other known species of the genus Enterobacter suggested that strain MSA2 could be a novel PGPB strain. In vitro studies were carried for the plant growth promoting attribute of this culture. It tested positive for ACC (1-aminocyclopropane-1-carboxylic acid) deaminase production, phytase, phosphate solubilization, IAA (Indole acetic acid) production, siderophore, and ammonia production. The isolate was then used as a inoculant for the vegetative study of Jatropha curcas plant. Enterobacter cancerogenus MSA2 supplemented with 1% carboxymethylcellulose showed overall plant growth promotion effect resulting in enhanced root length (124.14%), fresh root mass (81%), fresh shoot mass (120.02%), dry root mass (124%), dry shoot mass (105.54%), number of leaf (30.72%), chlorophyll content (50.41%), and biomass (87.20%) over control under the days of experimental observation. This study was designed for 120 days and was in triplicate and the data was collected at every 30 days.
一种新型的植物促生肠杆菌 MSA2 是一种从麻疯树的根际中分离出来的 γ-变形菌，麻疯树是一种有潜力的重要生物燃料原料植物。基于表型、生理、生化和系统发育研究，菌株 MSA2 可归类为肠杆菌属的一个成员。然而，与其他已知的肠杆菌属物种的特征比较表明，菌株 MSA2 可能是一种新型的植物促生菌。进行了体外研究以确定该培养物的植物促生特性。它对 ACC（1-氨基环丙烷-1-羧酸）脱氨酶生产、植酸酶、磷酸盐溶解、IAA（吲哚乙酸）生产、铁载体和氨生产呈阳性。然后，将该分离物用作麻疯树植物营养生长研究的接种物。肠杆菌属 MSA2 补充 1%羧甲基纤维素显示出整体的植物生长促进效果，导致根长（124.14%）、新鲜根质量（81%）、新鲜茎质量（120.02%）、干根质量（124%）、干茎质量（105.54%）、叶片数（30.72%）、叶绿素含量（50.41%）和生物量（87.20%）的增加，与对照相比，在实验观察的天数内。本研究设计为 120 天，重复 3 次，每 30 天收集一次数据。
