Microbiology Department, Biological Science Faculty, University of Concepción, P.O. Box 160-C, Correo 3, Concepción, Chile.
World J Microbiol Biotechnol. 2012 Apr;28(4):1511-21. doi: 10.1007/s11274-011-0953-6. Epub 2011 Nov 26.
The Patagonian Lakes have particular environmental conditions with or without intermittent disturbances. The study of the microorganisms present in aquatic ecosystems has increased notably because they can be used as micro-scale bioindicators of, among others, anthropogenic pollution and climatic change. The aim of the work was to compare the composition of the bacterial communities associated with sediments of three Patagonian Lakes with different geomorphologic patterns and disturbances. The lake sediments were characterized by molecular techniques, physiology profiles and physico-chemical analyses. The metabolic and physiological profiles of the microbial community demonstrated that non-impacted Tranquilo Lake is statistically different to impacted Bertrand and Plomo Lakes. Similar results were detected by DGGE profiles. FISH results demonstrated that betaproteobacteria showed the highest count in the Tranquilo Lake while gammaproteobacteria showed the highest counts in the Bertrand and Plomo Lakes, indicating that their sediments are highly dystrophic. The results demonstrate differences in the metabolic activity and structural and functional composition of bacterial communities of the studied lakes, which have different geomorphological patterns due to disturbances such as volcanic activity and the climatic change.
巴塔哥尼亚湖泊具有特殊的环境条件，或有间歇性干扰，或没有间歇性干扰。由于微生物可以作为人为污染和气候变化等的微尺度生物指标，因此对水生生态系统中存在的微生物的研究显著增加。这项工作的目的是比较具有不同地貌形态和干扰的三个巴塔哥尼亚湖泊沉积物中相关细菌群落的组成。通过分子技术、生理学谱和理化分析对湖泊沉积物进行了表征。微生物群落的代谢和生理特征表明，非受干扰的特兰奎洛湖在统计学上与受干扰的贝特朗湖和普洛莫湖不同。DGGE 谱也检测到了相似的结果。FISH 结果表明，β变形菌在特兰奎洛湖中数量最多，而γ变形菌在贝特朗湖和普洛莫湖中数量最多，这表明它们的沉积物高度贫营养化。研究结果表明，由于火山活动和气候变化等干扰，这些具有不同地貌形态的湖泊的细菌群落的代谢活性以及结构和功能组成存在差异。
