Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
World J Microbiol Biotechnol. 2012 May;28(5):2113-23. doi: 10.1007/s11274-012-1016-3. Epub 2012 Feb 9.
A collection of 70 Burkholderia cepacia complex isolates, recovered from clinical, water, and agricultural resources in China in our previous studies, were tested to assess their potential pathogenicity and association of biofilm formation with pathogenicity. The pathogenicity was tested in the alternative infection models alfalfa, detached lettuce midrib, Galleria mellonella (wax moth), rat agar bead, and lettuce intact leaves. Severe to moderate pathogenicity were observed for isolates of clinical and water origin compared to agricultural isolates, with the exception of a few clinical isolates exhibiting reduced pathogenicity. Virulent isolates persisted in rat lungs until 21 days post infection causing histopathological changes like inflammation, while in lettuce midrib tissues invasion, localization, and replication of bacteria were observed. Biofilm formation ability was also documented in high frequency among water and clinical virulent isolates compared to agricultural isolates. Although variations in pathogenicity were observed for a few isolates, results obtained from different model systems including lettuce were consistent. Our studies indicate that water and clinical isolates showed severe virulence and strong biofilm formation ability compared to agricultural isolates. The results also show lettuce as a promising infection model not only to study the pathogenicity factors used by Bcc bacteria but also for characterization the in vivo transcriptional profile for different niches adaptation of this opportunistic pathogen.
我们之前的研究中收集了 70 株洋葱伯克霍尔德氏菌复合群的分离株，这些分离株来自中国的临床、水和农业资源，我们对其进行了测试，以评估它们的潜在致病性和生物膜形成与致病性的关系。在苜蓿、离体生菜中肋、大蜡螟（白蜡虫）、大鼠琼脂珠和完整生菜叶片的替代感染模型中测试了致病性。与农业分离株相比，临床和水源分离株的致病性为严重至中度，除了少数临床分离株表现出降低的致病性。毒力分离株在感染后 21 天内在大鼠肺部持续存在，导致炎症等组织病理学变化，而在生菜中肋组织中观察到细菌的入侵、定位和复制。与农业分离株相比，水和临床毒力分离株也具有较高的生物膜形成能力。尽管一些分离株的致病性存在差异，但从不同模型系统（包括生菜）获得的结果是一致的。我们的研究表明，与农业分离株相比，水和临床分离株表现出严重的毒力和较强的生物膜形成能力。结果还表明，生菜不仅是研究 Bcc 细菌使用的致病性因素的有前途的感染模型，也是研究该机会性病原体不同生态位适应的体内转录谱的模型。
