Inoue Kengo, Habe Hiroshi, Yamane Hisakazu, Omori Toshio, Nojiri Hideaki
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FEMS Microbiol Lett. 2005 Apr 1;245(1):145-53. doi: 10.1016/j.femsle.2005.03.009.
Twenty-seven carbazole-utilizing bacterial strains were isolated from environmental samples, and were classified into 14 groups by amplified ribosomal DNA restriction analysis. Southern hybridization analyses showed that 3 and 17 isolates possessed the car gene homologs of Pseudomonas resinovorans CA10 and Sphingomonas sp. strain KA1, respectively. Of the 17 isolates, 2 isolates also have the homolog of the carAa gene of Sphingomonas sp. strain CB3. While the genome of one isolate, a Gram-positive Nocardioides sp. strain IC177, showed no hybridization to any car gene probes, PCR and sequence analyses indicated that strain IC177 had tandemly linked carAa and carC gene homologs whose deduced amino acid sequences showed 51% and 36% identities with those of strain KA1.
从环境样本中分离出27株利用咔唑的细菌菌株，并通过扩增核糖体DNA限制性分析将其分为14组。Southern杂交分析表明，分别有3株和17株分离株具有树脂糖假单胞菌CA10和鞘氨醇单胞菌属菌株KA1的car基因同源物。在这17株分离株中，有2株还具有鞘氨醇单胞菌属菌株CB3的carAa基因同源物。虽然一株革兰氏阳性诺卡氏菌属菌株IC177的基因组与任何car基因探针均无杂交，但PCR和序列分析表明，菌株IC177具有串联连接的carAa和carC基因同源物，其推导的氨基酸序列与菌株KA1的氨基酸序列具有51%和36%的同一性。
