Key Laboratory of Molecular Microbiology Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.
World J Microbiol Biotechnol. 2012 Oct;28(10):3039-52. doi: 10.1007/s11274-012-1114-2. Epub 2012 Jun 29.
The diversity and distribution of bacterial and archaeal communities in four different water flooding oil reservoirs with different geological properties were investigated using 16S rDNA clone library construction method. Canonical correspondence analysis was used to analyze microbial community clustering and the correlation with environmental factors. The results indicated that the diversity and abundance in the bacterial communities were significantly higher than the archaeal communities, while both of them had high similarity within the communities respectively. Phylogenetic analysis showed that of compositions of bacterial communities were distinctly different both at phylum and genus level. Proteobacteria dominated in each bacterial community, ranging from 61.35 to 75.83 %, in which α-proteobacteria and γ-proteobacteria were the main groups. In comparison to bacterial communities, the compositions of archaeal communities were similar at phylum level, while varied at genus level, and the dominant population was Methanomicrobia, ranging from 65.91 to 92.74 % in the single oil reservoir. The factor that most significantly influenced the microbial communities in these reservoirs was found to be temperature. Other environmental factors also influenced the microbial communities but not significantly. It is therefore assumed that microbial communities are formed by an accumulated effect of several factors. These results are essential for understanding ecological environment of the water flooding oil reservoirs and providing scientific guidance to the performance of MEOR technology.
采用 16S rDNA 克隆文库构建方法，研究了具有不同地质性质的 4 个不同注水油藏中细菌和古菌群落的多样性和分布。用典范对应分析方法分析了微生物群落聚类及其与环境因子的相关性。结果表明，细菌群落的多样性和丰度明显高于古菌群落，而两者在群落内均具有很高的相似性。系统发育分析表明，细菌群落的组成在门和属水平上均有明显差异。每个细菌群落都以变形菌门为主，占 61.35%75.83%，其中α-变形菌门和γ-变形菌门是主要类群。与细菌群落相比，古菌群落的组成在门水平上相似，而在属水平上则有所不同，优势种群是产甲烷菌，在单油藏中占 65.91%92.74%。对这些油藏中微生物群落影响最大的因素是温度。其他环境因素也影响微生物群落，但影响不显著。因此，可以假设微生物群落是由几个因素的累积效应形成的。这些结果对于了解注水油藏的生态环境以及为 MEOR 技术的应用提供科学指导具有重要意义。
