Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang 550025, China.
State Key Laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China.
Biomed Res Int. 2022 Aug 23;2022:3097521. doi: 10.1155/2022/3097521. eCollection 2022.
Protein elicitors play a key role in signaling or displaying plant defense mechanism and emerging as vital tools for biocontrol of insects. This study was aimed at the characterization of the novel protein elicitor isolated from entomopathogenic fungi (V3) strain and its activity against whitefly, , in cotton ( L.). The sequence of purified elicitor protein showed 100% similarity with hypothetical protein LEL_00878 (Cordyceps confragosa RCEF 1005) (GenBank accession no. OAA81333.1). This novel protein elicitor has 253 amino acid residues and 762 bp with a molecular mass of 29 kDa. Their combatant protein was expressed in using pET-28a (+) plasmid. Bioassay was revealed to quantify the impact of numerous concentrations of protein (i.e., 58.32, 41.22, and 35.41 g/ml) on the fecundity rate of on cotton plants. Bioassay results exhibited a significant effect ( ≤ 0.001) of all the concentrations of protein on the fecundity rate of . In addition, the gene expression analysis found a significant upregulation of the major genes associated with salicylic acid (SA) and jasmonic acid (JA) defense pathways in elicitor protein-treated plants. Our results showed that the potential application of novel protein elicitor derived from will be used as future biointensive controlling approaches against whitefly,
蛋白激发子在植物防御机制的信号转导或表现中起着关键作用，并且正在成为防治昆虫的重要工具。本研究旨在对从昆虫病原真菌（V3）菌株中分离出的新型蛋白激发子进行表征，并研究其对棉花（L.）烟粉虱的活性。纯化的激发子蛋白序列与假设蛋白 LEL_00878（Cordyceps confragosa RCEF 1005）（GenBank 登录号 OAA81333.1）具有 100%的相似性。这种新型蛋白激发子含有 253 个氨基酸残基和 762 bp，分子量为 29 kDa。它们的抗菌蛋白在大肠杆菌中使用 pET-28a(+)质粒进行表达。通过生物测定来量化不同浓度的蛋白（即 58.32、41.22 和 35.41 g/ml）对棉花植株上烟粉虱繁殖率的影响。生物测定结果表明，所有蛋白浓度对烟粉虱繁殖率均有显著影响（≤0.001）。此外，基因表达分析发现，在蛋白激发子处理的植物中，与水杨酸（SA）和茉莉酸（JA）防御途径相关的主要基因表达显著上调。我们的研究结果表明，来源于的新型蛋白激发子具有潜在的应用前景，可作为未来防治烟粉虱的生物强化控制方法。
