Sönnichsen M, Müller B W
Department of Pharmaceutics and Biopharmaceutics, Christian-Albrecht-University, Kiel, Germany.
Lipids. 1999 Dec;34(12):1347-9. doi: 10.1007/s11745-999-0488-z.
A quantitative method for the one-step esterification and determination of absolute amounts of polyunsaturated fatty acids in biotechnologically produced fungal mycelia is proposed. A system of two internal standards was used to quantify the total fatty acid content of the samples by gas chromatography. The degree of methylation of the analyzed fatty acids was calculated using the internal standards, and subsequently this quotient was used to validate the derivatization and extraction reactions. By utilizing this degree of methylation, the corrected amounts of the various fatty acids in the sample can be calculated.
提出了一种用于一步酯化和测定生物技术生产的真菌菌丝体中多不饱和脂肪酸绝对含量的定量方法。使用两种内标物的系统通过气相色谱法对样品中的总脂肪酸含量进行定量。利用内标物计算分析脂肪酸的甲基化程度，随后用该商值验证衍生化和提取反应。通过利用这种甲基化程度，可以计算样品中各种脂肪酸的校正量。
