Frisque R J, Martin J D, Padgett B L, Walker D L
J Virol. 1979 Nov;32(2):476-82. doi: 10.1128/JVI.32.2.476-482.1979.
The infectivity of JC virus DNA was demonstrated in its most permissive cell culture, primary human fetal glial cells. The amount of infectivity observed in these heterogeneous cultures varied considerably between batches of cells. Contrary to results obtained with the papovaviruses simian virus 40 and BK virus, the calcium technique (F. L. Graham and A. J. van der Eb, Virology 52:456--467, 1973) was found to be more efficient at promoting JC virus DNA infectivity than the DEAE-dextran method (J. H. McCutchan and J. S. Pagano, J. Natl. Cancer Inst. 41:351--357, 1968): maximum infectivity titers of 4 x 10-(4) and 6 x 10(3) fluorescent cell units per microgram of DNA, respectively. These values represent an approximate recovery of infectivity from virus of between 0.02 and 0.14%. Comparisons of infectivity of DNAs obtained from four isolates of JC virus and which differed in their degrees of heterogeneity did not reveal significant differences. The JC virus DNA was not infectious in primary human fetal lung and kidney cells.
JC病毒DNA的感染性在其最易感染的细胞培养物——原代人胎儿神经胶质细胞中得到了证实。在这些异质培养物中观察到的感染性数量在不同批次的细胞之间有很大差异。与用乳头多瘤空泡病毒猴病毒40和BK病毒获得的结果相反，发现钙技术（F. L. 格雷厄姆和A. J. 范德埃布，《病毒学》52:456 - 467，1973）在促进JC病毒DNA感染性方面比DEAE - 葡聚糖方法（J. H. 麦卡琴和J. S. 帕加诺，《国家癌症研究所杂志》41:351 - 357，1968）更有效：每微克DNA的最大感染性滴度分别为4×10⁻⁴和6×10³荧光细胞单位。这些值代表从病毒中感染性的近似回收率在0.02%至0.14%之间。对从四株JC病毒分离株获得的、异质性程度不同的DNA的感染性进行比较，未发现显著差异。JC病毒DNA在原代人胎儿肺和肾细胞中无感染性。
