用从肿瘤病毒SV40中提取的DNA对人二倍体细胞进行转化。
Human diploid cell transformation by DNA extracted from the tumor virus SV40.
作者信息
Aaronson S, Todaro S
出版信息
Science. 1969 Oct 17;166(3903):390-1. doi: 10.1126/science.166.3903.390.
PMID:4309480
Abstract
The DNA isolated from simian virus 40 (SV40) can transform human fibroblast cells in tissue culture. Clonal lines of DNA-transformed human cells have been obtained that have properties characteristic of cells transformed by whole virus. They all contain SV40 T-antigen, and infectious virus can be recovered by cocultivation. This is the first demonstration of a permanent genetic alteration produced in human cells by purified DNA.
摘要
从猴病毒40（SV40）中分离出的DNA能在组织培养中转化人成纤维细胞。现已获得DNA转化的人细胞克隆系，这些克隆系具有被完整病毒转化的细胞的特征特性。它们都含有SV40 T抗原，并且通过共培养可回收感染性病毒。这是首次证明纯化的DNA能在人细胞中产生永久性基因改变。
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