College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding, China.
Luminescence. 2013 Jul-Aug;28(4):592-6. doi: 10.1002/bio.2398. Epub 2012 Jul 15.
A novel chemiluminescence (CL) method was developed for the determination of cefazolin sodium based on the CL reaction between the Cu(HIO6)2 Cu(III) complex and luminol in alkaline solution. Results showed that CL emission of Cu(III) complex-luminol in alkaline medium was significantly different from that in acidic medium. A possible mechanism of the enhanced effect of cefazolin on CL emission of the Cu(HIO6)2-luminol system was proposed. The effect of the reaction conditions on CL emissions was examined. Under optimized conditions, a good linear relationship was obtained between CL intensity and concentrations of cefazolin sodium in the range of 2.0 x 10(-8) to 2.0 x 10(-6) g/mL with a correlation coefficient of R(2) = 0.9978. The limit of detection was 4.58 x 10(-9) g/mL. The proposed method was applied for the determination of cefazolin sodium in real samples with recoveries of 82.0-109% with an RSD of 0.7-2.1%. The proposed method was successfully used for the determination of cefazolin sodium in injectable powder preparations and human urine with satisfactory results.
建立了一种基于Cu(HIO6)2-Cu(III)配合物与碱性溶液中鲁米诺之间的化学发光(CL)反应测定头孢唑林钠的新方法。结果表明，碱性介质中 Cu(III)配合物-鲁米诺的 CL 发射与酸性介质明显不同。提出了头孢唑林增强Cu(HIO6)2-鲁米诺体系 CL 发射增强效应的可能机制。考察了反应条件对 CL 发射的影响。在优化条件下，头孢唑林钠在 2.0×10(-8)~2.0×10(-6)g/mL 范围内与 CL 强度呈良好的线性关系，相关系数为 R(2)=0.9978。检出限为 4.58×10(-9)g/mL。该方法用于实际样品中头孢唑林钠的测定，回收率为 82.0%-109%，相对标准偏差为 0.7%-2.1%。该方法成功用于注射用粉针制剂和人尿中头孢唑林钠的测定，结果令人满意。
